Human MMP-14 (Matrix Metalloproteinase 14) ELISA Kit
The Human MMP-14 (Matrix Metalloproteinase-14) ELISA Kit is specifically designed for the precise measurement of MMP-14 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.MMP-14 is a key enzyme involved in extracellular matrix remodeling, and its dysregulation is linked to various pathological conditions including cancer, tissue damage, and inflammatory diseases.
By detecting MMP-14 levels, researchers can gain valuable insights into disease mechanisms and potentially identify new therapeutic targets.Overall, the Human MMP-14 ELISA Kit from AssayGenie is a valuable tool for studying MMP-14 biology and its implications in disease, offering researchers a reliable and efficient method for quantifying MMP-14 levels in human samples.
Product Name:
Human MMP-14 (Matrix Metalloproteinase 14) ELISA Kit
SKU:
HUES03318
Target:
Human MMP-14 (Matrix Metalloproteinase 14)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-14. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMP-14. You can calculate the concentration of Human MMP-14 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
94-108
98-113
Average (%)
99
101
105
1:4
Range (%)
88-102
84-97
81-94
Average (%)
95
89
88
1:8
Range (%)
93-107
80-92
86-99
Average (%)
98
86
92
1:16
Range (%)
91-105
84-94
86-99
Average (%)
97
88
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
85-98
92
Cell culture media (n=5)
86-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.15
6.12
17.04
2.36
6.21
18.28
Standard deviation
0.14
0.34
0.78
0.12
0.33
0.67
C V (%)
6.51
5.56
4.58
5.08
5.31
3.67
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MMP-14 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-14 in samples. No significant cross-reactivity or interference between Human MMP-14 and analogues was observed.
