Human MIS12 Recombinant Protein (RPPB3976)
- SKU:
- RPPB3976
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- MIS12
- Synonyms:
- hMis12
- KNTC2AP
- MTW1
- Protein MIS12 homolog
- Source:
- Escherichia Coli
- Uniprot:
- Q9H081
Description
| Product Name: | Human MIS12 Recombinant Protein |
| Product Code: | RPPB3976 |
| Size: | 20µg |
| Species: | Human |
| Target: | MIS12 |
| Synonyms: | hMis12, KNTC2AP, MTW1, Protein MIS12 homolog. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | MIS12 protein solution (1mg/ml) containing 20mM Tris-HCl buffer, (pH 8.0) ,2M Urea and10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMSVDPMT YEAQFFGFTP QTCMLRIYIA FQDYLFEVMQ AVEQVILKKL DGIPDCDISP VQIRKCTEKF LCFMKGHFDN LFSKMEQLFL QLILRIPSNI LLPEDKCKET PYSEEDFQHL QKEIEQLQEK YKTELCTKQA LLAELEEQKI VQAKLKQTLT FFDELHNVGR DHGTSDFRES LVSLVQNSRK LQNIRDNVEK ESKRLKIS |
Protein MIS12 Belongs to the mis12 family. MIS12 is an element of the MIS12 complex, which is necessary for kinetochore formation during mitosis and normal chromosome alignment and segregation. The MIS12 complex which comprised of MIS12,DSN1 , NSL1 and PMF-1. MIS12 is section of a network of complexes that supply microtubule attachment and generates pulling forces from depolymerization.
MIS12 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 228 amino acids (1-205 a.a.) and having a molecular mass of 26.5kDa. MIS12 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MIS12: Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. Belongs to the mis12 family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 17p13.2 Cellular Component: nucleus; cytosol; MIS12/MIND type complex Molecular Function:protein binding Biological Process: mitosis; kinetochore assembly; cell division; mitotic cell cycle; chromosome segregation |
| UniProt Code: | Q9H081 |
| NCBI GenInfo Identifier: | 384551679 |
| NCBI Gene ID: | 79003 |
| NCBI Accession: | NP_076944.1 |
| UniProt Secondary Accession: | Q9H081,Q96N24, |
| UniProt Related Accession: | Q9H081 |
| Molecular Weight: | 24,140 Da |
| NCBI Full Name: | Homo sapiens MIS12 kinetochore complex component (MIS12), transcript variant 2, mRNA |
| NCBI Synonym Full Names: | MIS12 kinetochore complex component |
| NCBI Official Symbol: | MIS12 |
| NCBI Official Synonym Symbols: | MTW1; hMis12; KNTC2AP; 2510025F08Rik |
| NCBI Protein Information: | protein MIS12 homolog; MIS12, MIND kinetochore complex component, homolog; homolog of yeast Mis12 |
| UniProt Protein Name: | Protein MIS12 homolog |
| Protein Family: | Protein |
| UniProt Gene Name: | MIS12 |
| UniProt Entry Name: | MIS12_HUMAN |
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