Human MIF Recombinant Protein (RPPB0760)
- SKU:
- RPPB0760
- Product type:
- Recombinant Protein
- Size:
- 3x10ug
- Species:
- Human
- Target:
- MIF
- Synonyms:
- Phenylpyruvate tautomerase
- Glycosylation-inhibiting factor
- GIF
- MMIF
- Source:
- Escherichia Coli
- Uniprot:
- P14174
Description
| Product Name: | Human MIF Recombinant Protein |
| Product Code: | RPPB0760 |
| Size: | 25µg |
| Species: | Human |
| Target: | MIF |
| Synonyms: | Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, MMIF, MIF. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | MIF-Protein was lyophilized from 10mM sodium phosphate buffer pH-7.5. |
| Solubility: | It is recommended to reconstitute the lyophilized MIF-Protein in sterile 18M?-cm H2O at a concentration between 0.1mg-1mg per 1ml. |
| Stability: | Lyophilized MIF-protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MIF-protein should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | MPMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLMAFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVYINYYDMNAANVGWNNSTFA |
| Biological Activity: | Human PBMCs were cultured with 0 to 1000ng/ml Human MIF. Production of IL-8 was measured via ELISA after 24 hours. The ED50 which was found to be 88-132ng/ml. |
The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.
MIF human Recombinant was cloned into an E.coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.Macrophage Inducing Factor Human Recombinant is a single, non-glycosylated, polypeptide chain containing 115 amino acids and having a molecular mass of 12.5 kDa.
| UniProt Protein Function: | MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase. |
| UniProt Protein Details: | Protein type:EC 5.3.2.1; Isomerase; EC 5.3.3.12; Amino Acid Metabolism - tyrosine; Cell development/differentiation; Cytokine; Apoptosis; Amino Acid Metabolism - phenylalanine Chromosomal Location of Human Ortholog: 22q11.23 Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; myelin sheath; nucleoplasm; vesicle Molecular Function:chemoattractant activity; cytokine activity; dopachrome isomerase activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; phenylpyruvate tautomerase activity; protein binding; receptor binding Biological Process: blood coagulation; carboxylic acid metabolic process; cell aging; cell proliferation; cell surface receptor linked signal transduction; DNA damage response, signal transduction by p53 class mediator; inflammatory response; innate immune response; leukocyte migration; negative regulation of apoptosis; negative regulation of cellular protein metabolic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of mature B cell apoptosis; negative regulation of myeloid cell apoptosis; positive chemotaxis; positive regulation of B cell proliferation; positive regulation of cytokine secretion; positive regulation of fibroblast proliferation; positive regulation of lipopolysaccharide-mediated signaling pathway; positive regulation of MAP kinase activity; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; prostaglandin biosynthetic process; regulation of macrophage activation Disease: Rheumatoid Arthritis, Systemic Juvenile |
| NCBI Summary: | This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P14174 |
| NCBI GenInfo Identifier: | 1170955 |
| NCBI Gene ID: | 4282 |
| NCBI Accession: | P14174.4 |
| UniProt Secondary Accession: | P14174,Q2V4Y5, Q6FHV0, A5Z1R8, B2R4S3, |
| UniProt Related Accession: | P14174 |
| Molecular Weight: | 12,476 Da |
| NCBI Full Name: | Macrophage migration inhibitory factor |
| NCBI Synonym Full Names: | macrophage migration inhibitory factor (glycosylation-inhibiting factor) |
| NCBI Official Symbol: | MIF |
| NCBI Official Synonym Symbols: | GIF; GLIF; MMIF |
| NCBI Protein Information: | macrophage migration inhibitory factor |
| UniProt Protein Name: | Macrophage migration inhibitory factor |
| UniProt Synonym Protein Names: | Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase (EC:5.3.3.12); Phenylpyruvate tautomerase |
| Protein Family: | MIF-like protein |
| UniProt Gene Name: | MIF |
| UniProt Entry Name: | MIF_HUMAN |
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