MHC class I polypeptide-related sequence B; also known as MICB; is a heavily glycosylated protein serving as a ligand for the type I I receptor NKG2D. MICB shares 85% amino acid identity with MICA; a closely related protein; both of which contain three extracellular immunoglobulin-like domains; but without capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen; binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells; CD8+?β T cells; and ?? T cells on which the receptor is expressed. MICA/B are minimally expressed on normal cells; but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance; viral infections; and autoimmune diseases.
Product Name:
Human MICB Recombinant Protein (RPES3102)
Product Code:
RPES3102
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
PERB11.2
Accession:
NP_005922.2
Sequence:
Met 1-Gly 298
Fusion tag:
C-His & Fc
Activity:
Immobilized human His-NKG2D (78-216) at 10 µg/ml (100 ?l/well) can bind human MICB-Fch; The EC50 of human MICB-Fch is 15.9-37.1 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain of human MICB (NP_005922.2) (Met 1-Gly 298) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Purity:
> 98 % as determined by reducing SDS-PAGE.
Mol Mass:
59.5 kDa
AP Mol Mass:
80-90 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
