Human MIA Recombinant Protein (RPPB0768)
- SKU:
- RPPB0768
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- MIA
- Synonyms:
- Melanoma-derived growth regulatory protein precursor
- Cartilage-derived retinoic acid-sensitive protein
- CD-RAP
- MIA
- Source:
- Escherichia Coli
- Uniprot:
- Q16674
Description
| Product Name: | Human MIA Recombinant Protein |
| Product Code: | RPPB0768 |
| Size: | 20µg |
| Species: | Human |
| Target: | MIA |
| Synonyms: | Melanoma-derived growth regulatory protein precursor, Cartilage-derived retinoic acid-sensitive protein, CD-RAP, MIA. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein was lyophilized from a concentrated (1mg/ml) solution containing 20mM Potassium-phosphate pH=7 and 150mM potassium chloride. |
| Solubility: | It is recommended to reconstitute the lyophilized Melanoma Inhibitory Activity in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized MIA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MIA should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | Agrees with the sequence of native MIA human with an addition N-terminal Methionine residueMGPMPKLADRKLCADQECSSHPISMAVALQDYMAPDCRFLTIHRGQVVYVFSLKGRGRFLWGGSVQGDYYGDLAARLGYFPSSIVREDQTLKVDVKTDKWDFYCQ |
| Biological Activity: | The biological activity is calculated by the inhibiting effect on the invasion of Mel In Tumor cells and found active in Mel In assay. |
The Melanoma Inhibitory protein (MIA) was identified as an inhibitor of in vitro growth of malignant melanoma cells. The protein contains a SH3 domain. MIA acts as a potent tumor cell growth inhibitor for malignant melanoma cells and some other neuroectodermal tumors, including gliomas, in an autocrine fashion. In a study of human melanoma cell lines with different metastatic capacity MIA mRNA expression appeared to be inversely correlated with pigmentation. MIA has been shown to represent a very sensitive and specific serum marker for systemic malignant melanoma that might be useful for staging of primary melanomas, detection of progression from localized to metastatic disease during follow-up, and monitoring therapy of advanced melanomas.
Melanoma Inhibitory Activity Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain consisting of 108 amino having a total molecular mass of 12237 Dalton.The MIA is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MIA: Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas. Belongs to the MIA/OTOR family.Protein type: Cell adhesion; Secreted, signal peptide; SecretedChromosomal Location of Human Ortholog: 19q13.2Cellular Component: extracellular spaceMolecular Function: growth factor activityBiological Process: cell proliferation |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q16674 |
| NCBI GenInfo Identifier: | 2498559 |
| NCBI Gene ID: | 8190 |
| NCBI Accession: | Q16674.1 |
| UniProt Secondary Accession: | Q16674,Q6FHV3 |
| UniProt Related Accession: | Q16674 |
| Molecular Weight: | 6,361 Da |
| NCBI Full Name: | Melanoma-derived growth regulatory protein |
| NCBI Synonym Full Names: | melanoma inhibitory activity |
| NCBI Official Symbol: | MIA |
| NCBI Official Synonym Symbols: | CD-RAP |
| NCBI Protein Information: | melanoma-derived growth regulatory protein |
| UniProt Protein Name: | Melanoma-derived growth regulatory protein |
| UniProt Synonym Protein Names: | Melanoma inhibitory activity protein |
| Protein Family: | Melanoma-derived growth regulatory protein |
| UniProt Gene Name: | MIA |
| UniProt Entry Name: | MIA_HUMAN |
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