Human MHCC/HLA-C (Major Histocompatibility Complex Class I C) CLIA Kit
The Human MHCC/HLA-C (Major Histocompatibility Complex Class I C) CLIA Kit is a cutting-edge diagnostic tool designed to accurately measure MHCC/HLA-C levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications. MHCC/HLA-C is a key component of the immune system, playing a crucial role in antigen presentation and immune response regulation. Dysregulation of MHCC/HLA-C has been linked to autoimmune diseases, infectious diseases, and transplant rejection, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
Trust in the reliability and accuracy of the Human MHCC/HLA-C CLIA Kit to advance your research and gain valuable insights into the intricate mechanisms of the immune system. Order yours today and unlock the potential for groundbreaking discoveries in immunology and disease research.
Product Name:
Human MHCC/HLA-C (Major Histocompatibility Complex Class I C) CLIA Kit
SKU:
HUES01207
Target:
Human MHCC/HLA-C (Major Histocompatibility Complex Class I C)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MHCC/HLA-C. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MHCC/HLA-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MHCC/HLA-C, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MHCC/HLA-C. You can calculate the concentration of Human MHCC/HLA-C in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
96-111
86-100
Average (%)
106
102
93
1:4
Range (%)
95-108
90-101
93-106
Average (%)
101
96
99
1:8
Range (%)
100-117
101-119
101-119
Average (%)
107
109
108
1:16
Range (%)
93-108
89-102
88-102
Average (%)
101
96
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-99
91
EDTA plasma (n=5)
89-104
95
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
213.59
630.82
1886.59
223.47
649.71
1780.21
Standard deviation
24.61
68.57
199.6
21.56
49.12
193.33
C V (%)
11.52
10.87
10.58
9.65
7.56
10.86
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MHCC/HLA-C concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MHCC/HLA-C in samples. No significant cross-reactivity or interference between Human MHCC/HLA-C and analogues was observed.
