The Human MGP (Matrix Gla Protein) CLIA Kit is specifically designed for the quantitative measurement of MGP levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for a variety of research applications. MGP is a key protein involved in regulating vascular calcification and promoting cardiovascular health. Dysregulation of MGP has been linked to various diseases such as atherosclerosis and kidney disease, making it an important biomarker for studying these conditions and developing potential therapeutic interventions.
This CLIA kit from Assay Genie offers researchers a powerful tool for investigating the role of MGP in health and disease, providing valuable insights into potential diagnostic and treatment strategies. With its easy-to-use format and robust performance, the Human MGP CLIA Kit is a valuable addition to any laboratory working in the field of cardiovascular research.
Product Name:
Human MGP (Matrix Gla Protein) CLIA Kit
SKU:
HUES00817
Target:
Human MGP (Matrix Gla Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MGP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MGP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MGP. You can calculate the concentration of Human MGP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-118
89-101
90-106
Average (%)
107
96
97
1:4
Range (%)
98-112
92-106
93-106
Average (%)
104
98
99
1:8
Range (%)
95-110
99-117
96-110
Average (%)
101
107
104
1:16
Range (%)
95-110
97-110
99-115
Average (%)
101
104
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
91
EDTA plasma (n=5)
88-101
94
Cell culture media (n=5)
86-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
22.89
79.32
204.58
22.05
86.53
219.41
Standard deviation
2.63
9.26
21.17
2.1
9.82
18.32
C V (%)
11.49
11.67
10.35
9.52
11.35
8.35
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MGP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MGP in samples. No significant cross-reactivity or interference between Human MGP and analogues was observed.
