The Human MFI2 (Melanotransferrin) CLIA Kit is a highly sensitive and specific assay designed for the precise measurement of melanotransferrin levels in human serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it an ideal tool for various research applications.Melanotransferrin is a glycoprotein that plays a crucial role in iron metabolism and cellular processes.
It is also implicated in various diseases, including cancer and neurodegenerative disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic strategies.Overall, the Human MFI2 (Melanotransferrin) CLIA Kit provides researchers with a powerful tool for accurately measuring melanotransferrin levels and advancing our understanding of its role in health and disease.
Product Name:
Human MFI2 (Melanotransferrin) CLIA Kit
SKU:
HUES01252
Target:
Human MFI2 (Melanotransferrin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MFI2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MFI2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MFI2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MFI2. You can calculate the concentration of Human MFI2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
100-116
102-116
Average (%)
106
108
108
1:4
Range (%)
96-109
96-109
95-108
Average (%)
102
102
100
1:8
Range (%)
87-100
90-103
99-114
Average (%)
93
96
106
1:16
Range (%)
87-98
94-108
86-99
Average (%)
93
102
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
100
EDTA plasma (n=5)
98-112
104
Cell culture media (n=5)
85-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.11
117.57
485.24
51.47
127.97
446.54
Standard deviation
5.51
12.16
31.1
6.26
13.41
38.89
C V (%)
11.45
10.34
6.41
12.16
10.48
8.71
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MFI2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MFI2 in samples. No significant cross-reactivity or interference between Human MFI2 and analogues was observed.
