Human MED4 Recombinant Protein (RPPB3951)
- SKU:
- RPPB3951
- Product type:
- Recombinant Protein
- Size:
- 25ug
- Species:
- Human
- Target:
- MED4
- Synonyms:
- Mediator of RNA polymerase II transcription subunit 4
- Mediator complex subunit 4
- Vitamin D3 receptor-interacting protein complex 36 kDa component
- Activator-recruited cofactor 36 kDa component
- Source:
- Escherichia Coli
- Uniprot:
- Q9NPJ6
Description
| Product Name: | Human MED4 Recombinant Protein |
| Product Code: | RPPB3951 |
| Size: | 25µg |
| Species: | Human |
| Target: | MED4 |
| Synonyms: | Mediator of RNA polymerase II transcription subunit 4, Mediator complex subunit 4, Vitamin D3 receptor-interacting protein complex 36 kDa component, Activator-recruited cofactor 36 kDa component, TRAP/SMCC/PC2 subunit p36 subunit, DRIP36, ARC36, MED4, VDRIP, TRAP36, HSPC126, FLJ10956, RP11-90M2.2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The MED4 protein solution contains 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 1mM DTT and 100mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 80.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MAASSSGEKE KERLGGGLGV AGGNSTRERL LSALEDLEVL SRELIEMLAI SRNQKLLQAG EENQVLELLI HRDGEFQELM KLALNQGKIH HEMQVLEKEV EKRDGDIQQL QKQLKEAEQI LATAVYQAKE KLKSIEKARK GAISSEEIIK YAHRISASNA VCAPLTWVPG DPRRPYPTDL EMRSGLLGQM NNPSTNGVNG HLPGDALAAG RLPDVLAPQY PWQSNDMSMN MLPPNHSSDF LLEPPGHNKE DEDDVEIMST DSSSSSSESD LEHHHHHH |
Mediator complex subunit 4 (MED4) is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED4 is a component of the vitamin D receptor-interacting protein (DRIP) complex which functions as a nuclear receptor coactivator. The DRIP complex is able to activate nuclear receptors in a ligand-dependent manner. MED4 functions as a link to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription apparatus. MED4 is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
MED4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 278 amino acids (1-270 a.a.) and having a molecular mass of 30.7kDa.MED4 is fused to 8 amino acids His Tag at C-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MED4: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 4 family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 13q14.2 Cellular Component: nucleoplasm; membrane; Srb-mediator complex; nucleus Molecular Function:protein binding; ligand-dependent nuclear receptor transcription coactivator activity; vitamin D receptor binding; transcription cofactor activity; receptor activity; thyroid hormone receptor binding Biological Process: steroid hormone receptor signaling pathway; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; gene expression |
| NCBI Summary: | This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012] |
| UniProt Code: | Q9NPJ6 |
| NCBI GenInfo Identifier: | 29840770 |
| NCBI Gene ID: | 29079 |
| NCBI Accession: | Q9NPJ6.1 |
| UniProt Secondary Accession: | Q9NPJ6,Q53GB4, Q53H68, Q5T912, Q6FHC4, Q6IA79, Q9BS95 Q9NYR5, B4DX67, |
| UniProt Related Accession: | Q9NPJ6 |
| Molecular Weight: | 24,947 Da |
| NCBI Full Name: | Mediator of RNA polymerase II transcription subunit 4 |
| NCBI Synonym Full Names: | mediator complex subunit 4 |
| NCBI Official Symbol: | MED4 |
| NCBI Official Synonym Symbols: | ARC36; VDRIP; DRIP36; TRAP36; HSPC126 |
| NCBI Protein Information: | mediator of RNA polymerase II transcription subunit 4; TRAP/SMCC/PC2 subunit p36; mediator, 34-kD subunit, homolog; activator-recruited cofactor 36 kDa component; vitamin D receptor-interacting protein, 36-kD; vitamin D3 receptor-interacting protein complex 36 kDa component |
| UniProt Protein Name: | Mediator of RNA polymerase II transcription subunit 4 |
| UniProt Synonym Protein Names: | Activator-recruited cofactor 36 kDa component; ARC36; Mediator complex subunit 4; TRAP/SMCC/PC2 subunit p36 subunit; Vitamin D3 receptor-interacting protein complex 36 kDa component; DRIP36 |
| Protein Family: | Mediator of RNA polymerase II transcription |
| UniProt Gene Name: | MED4 |
| UniProt Entry Name: | MED4_HUMAN |
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