Human MCSF (Macrophage Colony Stimulating Factor 1) ELISA Kit
The Human MCSF (Macrophage Colony Stimulating Factor 1) ELISA Kit is specifically designed for the precise quantification of MCSF levels in human serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and reproducible results, making it an indispensable tool for a wide range of research applications.MCSF is a key signaling protein that plays a critical role in the regulation of macrophage function and differentiation. It is involved in various biological processes, including immune responses, inflammation, and tissue repair.
Abnormal MCSF levels have been linked to diseases such as cancer, autoimmune disorders, and inflammatory conditions, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.Overall, the Human MCSF ELISA Kit offers researchers a reliable and efficient method for studying the role of MCSF in various physiological and pathological processes, facilitating advancements in biomedical research and drug discovery.
Product Name:
Human MCSF (Macrophage Colony Stimulating Factor 1) ELISA Kit
SKU:
HUES01383
Target:
Human MCSF (Macrophage Colony Stimulating Factor 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MCSF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MCSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MCSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MCSF. You can calculate the concentration of Human MCSF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
90-107
85-97
Average (%)
92
97
92
1:4
Range (%)
88-102
85-100
84-98
Average (%)
94
92
90
1:8
Range (%)
91-107
84-94
84-96
Average (%)
98
89
89
1:16
Range (%)
89-104
82-93
82-94
Average (%)
96
88
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
91
EDTA plasma (n=5)
85-98
90
Cell culture media (n=5)
85-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.07
270.27
679.52
100.99
263.75
714.11
Standard deviation
5.21
12.19
35.74
5.59
11.34
31.85
C V (%)
5.54
4.51
5.26
5.54
4.3
4.46
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MCSF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MCSF in samples. No significant cross-reactivity or interference between Human MCSF and analogues was observed.
