Human MCP-4/CCL13 (Monocyte Chemotactic Protein 4) CLIA Kit
The Human MCP-4 (CCL13) Monocyte Chemotactic Protein 4 CLIA Kit is a cutting-edge tool designed to accurately measure levels of MCP-4 in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers precise and reliable results, making it suitable for a wide range of research applications.MCP-4, also known as CCL13, is a key chemokine involved in immune response and inflammation, playing a critical role in recruiting monocytes to sites of injury or infection.
Dysregulation of MCP-4 has been linked to various inflammatory diseases, making it a valuable biomarker for studying these conditions and developing targeted therapies.With the Human MCP-4 Monocyte Chemotactic Protein 4 CLIA Kit, researchers can gain deeper insights into the role of MCP-4 in health and disease, paving the way for advancements in diagnostics and treatment strategies.
Product Name:
Human MCP-4/CCL13 (Monocyte Chemotactic Protein 4) CLIA Kit
SKU:
HUES00670
Target:
Human MCP-4/CCL13 (Monocyte Chemotactic Protein 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MCP-4/CCL13. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MCP-4/CCL13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MCP-4/CCL13, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MCP-4/CCL13. You can calculate the concentration of Human MCP-4/CCL13 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
97-109
95-112
Average (%)
97
102
103
1:4
Range (%)
87-103
94-106
99-110
Average (%)
94
100
105
1:8
Range (%)
84-95
93-107
85-96
Average (%)
90
98
90
1:16
Range (%)
88-100
93-104
96-110
Average (%)
93
99
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
91
EDTA plasma (n=5)
96-111
104
Cell culture media (n=5)
100-114
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.15
45.2
168.45
24.84
40.99
182.55
Standard deviation
3.23
3.93
11.62
2.92
2.87
12.4
C V (%)
11.9
8.69
6.9
11.76
7.0
6.79
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MCP-4/CCL13 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MCP-4/CCL13 in samples. No significant cross-reactivity or interference between Human MCP-4/CCL13 and analogues was observed.
