Human MCM7 Recombinant Protein (RPPB3946)

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SKU:
RPPB3946
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
MCM7
Synonyms:
Minichromosome Maintenance Complex Component 7
Synonyms:
MCM7 Minichromosome Maintenance Deficient 7 (S Cerevisiae)
Synonyms:
Minichromosome Maintenance Deficient (S Cerevisiae) 7
Synonyms:
DNA Replication Licensing Factor MCM7
Source:
Escherichia Coli
Uniprot:
P33993
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Description

Product Name:Human MCM7 Recombinant Protein
Product Code:RPPB3946
Size:20µg
Species:Human
Target:MCM7
Synonyms:Minichromosome Maintenance Complex Component 7, MCM7 Minichromosome Maintenance Deficient 7 (S. Cerevisiae), Minichromosome Maintenance Deficient (S. Cerevisiae) 7, DNA Replication Licensing Factor MCM7, Homolog of S. Cerevisiae Cdc47, CDC47 Homolog, P1CDC47, PNAS146, P85MCM, MCM2, CDC47, P1.1-MCM3, EC 3.6.4.12.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The MCM7 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMVVATYT CDQCGAETYQ PIQSPTFMPL IMCPSQECQT NRSGGRLYLQ TRGSRFIKFQ EMKMQEHSDQ VPVGNIPRSI TVLVEGENTR IAQPGDHVSV TGIFLPILRT GFRQVVQGLL SETYLEAHRI VKMNKSEDDE SGAGELTREE LRQIAEEDFY EKLAASIAPE IYGHEDVKKA LLLLLVGGVD QSPRGMKIRG NINICLMGDP GVAKSQLLSY IDRLAPRSQY TTGRGSSGVG LTAAVLRDSV SGELTLEGGA LVLADQGVCC IDEFDKMAEA DRTAIHEVME QQTISIAKAG ILTTLNARCS ILAAANPAYG RYNPRRSLEQ NIQLPAALLS RFDLLWLIQD RPDRDNDLRL AQHITYVHQH SRQPPSQFEP LDMKLMRRYI AMCREKQPMV PESLADYITA AYVEMRR

MCM7 is a highly conserved mini-chromosome maintenance protein (MCM) vital for eukaryotic genome replication initiation. The MCM proteins form a hexameric protein complex which is a key component of the pre-replication complex (pre_RC) which takes part in replication forks formation and in DNA replication related proteins recruitment. The MCM complex is comprised of MCM2, 4, 6 and 7 proteins and possesses DNA helicase activity, such as DNA unwinding. 

MCM7 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 437 amino acids (1-414) and having a molecular mass of 48.6 kDa. MCM7 is fused to a 23 amino acid His-tag at N-terminus.

UniProt Protein Function:MCM7: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6- MCM4-MCM7-MCM3-MCM5 (By simililarity). Interacts with the ATR- ATRIP complex and with RAD17. Interacts with TIPIN. Interacts with MCMBP. Belongs to the MCM family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA replication; EC 3.6.4.12; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: chromatin; cytoplasm; MCM complex; membrane; nuclear chromosome, telomeric region; nucleoplasm

Molecular Function:ATP-dependent DNA helicase activity; DNA binding; protein binding

Biological Process: DNA replication; G1/S transition of mitotic cell cycle; regulation of phosphorylation; response to DNA damage stimulus

NCBI Summary:The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:P33993
NCBI GenInfo Identifier:20981696
NCBI Gene ID:4176
NCBI Accession:P33993.4
UniProt Secondary Accession:P33993,Q15076, Q96D34, Q96GL1, A4D2A1, A4D2A2, E9PGN9
UniProt Related Accession:P33993
Molecular Weight:60,643 Da
NCBI Full Name:DNA replication licensing factor MCM7
NCBI Synonym Full Names:minichromosome maintenance complex component 7
NCBI Official Symbol:MCM7  
NCBI Official Synonym Symbols:MCM2; CDC47; P85MCM; P1CDC47; PNAS146; PPP1R104; P1.1-MCM3  
NCBI Protein Information:DNA replication licensing factor MCM7
UniProt Protein Name:DNA replication licensing factor MCM7
UniProt Synonym Protein Names:CDC47 homolog; P1.1-MCM3
Protein Family:DNA replication licensing factor
UniProt Gene Name:MCM7  
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