Human MAPtau (Microtubule Associated Protein Tau/Tau Protein) ELISA Kit
The Human MAPTau (Microtubule-Associated Protein Tau) ELISA Kit is specifically designed for the precise measurement of MAPTau levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides high sensitivity and accuracy, ensuring dependable and consistent results for various research purposes.MAPTau is a key protein involved in stabilizing microtubules in cells and is known for its significance in neurodegenerative diseases such as Alzheimer's.
By measuring MAPTau levels, researchers can gain valuable insights into disease progression and potential therapeutic targets. This ELISA kit is therefore essential for studying the role of MAPTau in neurological disorders and advancing research towards effective treatments.
Product Name:
Human MAPtau (Microtubule Associated Protein Tau/Tau Protein) ELISA Kit
SKU:
HUES02072
Target:
Human MAPtau (Microtubule Associated Protein Tau/Tau Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MAPτ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MAPτ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MAPτ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MAPτ. You can calculate the concentration of Human MAPτ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
90-102
90-105
Average (%)
96
97
96
1:4
Range (%)
93-106
87-102
82-92
Average (%)
98
93
87
1:8
Range (%)
90-104
82-93
80-94
Average (%)
98
87
87
1:16
Range (%)
87-103
81-90
83-95
Average (%)
94
86
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
91
EDTA plasma (n=5)
94-108
99
Cell culture media (n=5)
90-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.8
52.53
178.71
24.88
56.27
182.54
Standard deviation
1.59
2.25
7.4
1.53
2.97
5.57
C V (%)
6.68
4.28
4.14
6.15
5.28
3.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MAPτ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MAPτ in samples. No significant cross-reactivity or interference between Human MAPτ and analogues was observed.
