Human MAP2K1 Recombinant Protein (RPPB2563)
- SKU:
- RPPB2563
- Product type:
- Recombinant Protein
- Size:
- 5ug
- Species:
- Human
- Target:
- MAP2K1
- Synonyms:
- MAP2K1
- MEK1
- PRKMK1
- MKK1
- Source:
- Sf9 Insect cells
- Uniprot:
- Q02750
Description
| Product Name: | Human MAP2K1 Recombinant Protein |
| Product Code: | RPPB2563 |
| Size: | 5µg |
| Species: | Human |
| Target: | MAP2K1 |
| Synonyms: | MAP2K1, MEK1, PRKMK1, MKK1, MAPKK 1, MAP kinase kinase 1. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | MAP2K1 protein solution (0.25mg/ml) contains 10% glycerol & Phosphate Buffered Saline (pH 7.4). |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADPMPKKKPT PIQLNPAPDG SAVNGTSSAE TNLEALQKKL EELELDEQQR KRLEAFLTQK QKVGELKDDD FEKISELGAG NGGVVFKVSH KPSGLVMARK LIHLEIKPAI RNQIIRELQV LHECNSPYIV GFYGAFYSDG EISICMEHMD GGSLDQVLKK AGRIPEQILG KVSIAVIKGL TYLREKHKIM HRDVKPSNIL VNSRGEIKLC DFGVSGQLID SMANSFVGTR SYMSPERLQG THYSVQSDIW SMGLSLVEMA VGRYPIPPPD AKELELMFGC QVEGDAAETP PRPRTPGRPL SSYGMDSRPP MAIFELLDYI VNEPPPKLPS GVFSLEFQDF VNKCLIKNPA ERADLKQLMV HAFIKRSDAE EVDFAGWLCS TIGLNQPSTP THAAGVHHHH HH |
The MAP2K1 protein is encoded by the MAP2K1 gene. This enzyme serves as a MAP (mitogen activated protein) kinase, it is a part of the dual specificity protein kinase family. Extracellular signal-regulated kinases such as MAP kinases has an important role in assimilation of various biochemical signals. MAP2K1 is located upstream to the MAP kinases and activates them by multiple intra and extracellular signals. The enzyme serves as a key factor in the signal transduction pathway of MAP kinase, therefore it takes part in the cell development (transcription regulation, proliferation, differentiation etc.).
MAP2K1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 402 amino acids (1-393a.a.) and having a molecular mass of 44.5kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).MAP2K1 is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MEK1: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf, Mos and Cot kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK1 and ERK2. An essential component of MAP kinase signal transduction pathways involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. |
| UniProt Protein Details: | Protein type:EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); Kinase, protein; Protein kinase, STE; STE group; STE7 family Chromosomal Location of Human Ortholog: 15q22.1-q22.33 Cellular Component: dendrite cytoplasm; Golgi apparatus; microtubule; focal adhesion; mitochondrion; endoplasmic reticulum; early endosome; perikaryon; cell cortex; cytosol; axon; perinuclear region of cytoplasm; late endosome; cytoplasm; plasma membrane; microtubule organizing center; nucleus Molecular Function:MAP kinase kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activator activity; protein binding; Ras GTPase binding; receptor signaling protein tyrosine phosphatase activity; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; mitogen-activated protein kinase kinase kinase binding; ATP binding; protein kinase activity Biological Process: axon guidance; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; activation of MAPK activity; protein heterooligomerization; negative regulation of homotypic cell-cell adhesion; response to glucocorticoid stimulus; stress-activated MAPK cascade; cell motility involved in cell locomotion; pathogenesis; toll-like receptor 3 signaling pathway; signal transduction; chemotaxis; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; neuron differentiation; negative regulation of cell proliferation; positive regulation of RNA elongation from RNA polymerase II promoter; small GTPase mediated signal transduction; vesicle transport along microtubule; response to axon injury; cell cycle arrest; toll-like receptor 4 signaling pathway; Golgi inheritance; epidermal growth factor receptor signaling pathway; mitosis; fibroblast growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; melanosome transport; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; keratinocyte differentiation; cell proliferation; regulation of stress-activated MAPK cascade; regulation of vascular smooth muscle contraction; Ras protein signal transduction; insulin receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; response to oxidative stress; positive regulation of Ras protein signal transduction; cell motility; positive regulation of cell differentiation; vascular endothelial growth factor receptor signaling pathway; positive regulation of cell migration Disease: Noonan Syndrome 1; Cardiofaciocutaneous Syndrome 3 |
| NCBI Summary: | The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q02750 |
| NCBI GenInfo Identifier: | 400274 |
| NCBI Gene ID: | 5604 |
| NCBI Accession: | Q02750.2 |
| UniProt Related Accession: | Q02750 |
| Molecular Weight: | 393 |
| NCBI Full Name: | Dual specificity mitogen-activated protein kinase kinase 1 |
| NCBI Synonym Full Names: | mitogen-activated protein kinase kinase 1 |
| NCBI Official Symbol: | MAP2K1 |
| NCBI Official Synonym Symbols: | CFC3; MEK1; MKK1; MAPKK1; PRKMK1 |
| NCBI Protein Information: | dual specificity mitogen-activated protein kinase kinase 1; MEK 1; MAPKK 1; MAPK/ERK kinase 1; ERK activator kinase 1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) |
| UniProt Protein Name: | Dual specificity mitogen-activated protein kinase kinase 1 |
| UniProt Synonym Protein Names: | ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1 |
| UniProt Gene Name: | MAP2K1 |
| UniProt Entry Name: | MP2K1_HUMAN |
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