Human MAP-2 (Microtubule Associated Protein 2) CLIA Kit
The Human MAP-2 (Microtubule-Associated Protein 2) CLIA Kit is a highly reliable and accurate assay designed for the quantitative detection of MAP-2 levels in human samples. This kit offers superior sensitivity and specificity, allowing for precise and reproducible results in serum, plasma, and cell culture supernatants.MAP-2 is a key protein that plays a critical role in the organization of microtubules in neurons, contributing to neuronal development and synaptic plasticity. Dysregulation of MAP-2 has been associated with various neurological disorders such as Alzheimer's disease and schizophrenia, making it an important biomarker for studying these conditions and potential therapeutic interventions.
With its advanced technology and user-friendly protocol, the Human MAP-2 CLIA Kit is an essential tool for researchers and clinicians studying neurodegenerative diseases and seeking to understand the intricate mechanisms of neuronal function. Trust in the accuracy and reliability of this kit for your research needs.
Product Name:
Human MAP-2 (Microtubule Associated Protein 2) CLIA Kit
SKU:
HUES01095
Target:
Human MAP-2 (Microtubule Associated Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MAP-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MAP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MAP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MAP-2. You can calculate the concentration of Human MAP-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
83-99
90-105
Average (%)
96
90
96
1:4
Range (%)
90-101
93-108
100-111
Average (%)
95
99
105
1:8
Range (%)
88-100
101-116
98-109
Average (%)
93
109
103
1:16
Range (%)
89-101
99-113
88-102
Average (%)
95
105
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-111
103
EDTA plasma (n=5)
92-102
97
Cell culture media (n=5)
84-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
189.75
558.35
1717.73
181.58
577.2
1888.36
Standard deviation
16.38
41.49
125.22
22.24
67.88
200.92
C V (%)
8.63
7.43
7.29
12.25
11.76
10.64
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MAP-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MAP-2 in samples. No significant cross-reactivity or interference between Human MAP-2 and analogues was observed.
