Human MAFF Recombinant Protein (RPPB3907)
- SKU:
- RPPB3907
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- MAFF
- Synonyms:
- hMafF
- Transcription factor MafF
- Source:
- Escherichia Coli
- Uniprot:
- Q9ULX9
Description
| Product Name: | Human MAFF Recombinant Protein |
| Product Code: | RPPB3907 |
| Size: | 20µg |
| Species: | Human |
| Target: | MAFF |
| Synonyms: | hMafF, U-MAF, Transcription factor MafF, U-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog F, MAFF. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The MAFF solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.4M Urea. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMSVDPLS SKALKIKREL SENTPHLSDE ALMGLSVREL NRHLRGLSAE EVTRLKQRRR TLKNRGYAAS CRVKRVCQKE ELQKQKSELE REVDKLAREN AAMRLELDAL RGKCEALQGF ARSVAAARGP ATLVAPASVI TIVKSTPGSG SGPAHGPDPA HGPASCS |
V-maf Musculoaponeurotic Fibrosarcoma Oncogene F (MAFF) which is expressed in the term myometrium and kidney is a basic leucine zipper (bZIP) transcription factor which lacks a transactivation domain. MAFF is known to bind the US-2 DNA element and probably heterodimerizes along with other leucine zipper-containing proteins to enhance expression of the OTR gene throughout pregnancy period. MAFF also forms homodimers, and since it lacks a transactivation domain, the homodimer operates as a repressor of transcription.
MAFF Human Recombinant produced in E. coli is a single polypeptide chain containing 187 amino acids (1-164) and having a molecular mass of 20.1kDa. MAFF is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MAFF: Interacts with the upstream promoter region of the oxytocin receptor gene. May be a transcriptional enhancer in the up-regulation of the oxytocin receptor gene at parturition. Since it lacks a putative transactivation domain, it may behave as a transcriptional repressor when it dimerize among himself. May also serve as a transcriptional activator by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. May be involved in the cellular stress response. Belongs to the bZIP family. Maf subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: mitochondrion; nucleoplasm; nucleus Biological Process: blood coagulation; parturition; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter |
| NCBI Summary: | The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2009] |
| UniProt Code: | Q9ULX9 |
| NCBI GenInfo Identifier: | 21542145 |
| NCBI Gene ID: | 23764 |
| NCBI Accession: | Q9ULX9.2 |
| UniProt Secondary Accession: | Q9ULX9,Q9Y525, B4DV49, |
| UniProt Related Accession: | Q9ULX9 |
| Molecular Weight: | 14,569 Da |
| NCBI Full Name: | Transcription factor MafF |
| NCBI Synonym Full Names: | MAF bZIP transcription factor F |
| NCBI Official Symbol: | MAFF |
| NCBI Official Synonym Symbols: | U-MAF; hMafF |
| NCBI Protein Information: | transcription factor MafF |
| UniProt Protein Name: | Transcription factor MafF |
| UniProt Synonym Protein Names: | U-Maf; V-maf musculoaponeurotic fibrosarcoma oncogene homolog F |
| Protein Family: | MaFF-interacting protein |
| UniProt Gene Name: | MAFF |
| UniProt Entry Name: | MAFF_HUMAN |
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