The Human MAC (Membrane Attack Complex) ELISA Kit is specifically designed for the precise detection of levels of MAC in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an invaluable tool for various research applications.MAC is a critical component of the immune system's complement pathway, involved in target cell lysis and immune defense against pathogens.
Dysregulation of MAC has been associated with autoimmune diseases, inflammatory disorders, and infections, highlighting its importance as a biomarker for understanding immune response and developing therapeutic interventions.By utilizing the Human MAC ELISA Kit, researchers can gain valuable insights into the role of MAC in immune regulation and disease pathology, paving the way for innovative diagnostic and treatment strategies.
Product Name:
Human MAC (Membrane Attack Complex) ELISA Kit
SKU:
HUES03421
Target:
Human MAC (Membrane Attack Complex)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MAC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MAC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MAC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MAC. You can calculate the concentration of Human MAC in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
87-98
84-97
Average (%)
100
93
91
1:4
Range (%)
88-101
85-98
84-95
Average (%)
95
92
90
1:8
Range (%)
86-101
81-93
86-97
Average (%)
92
87
92
1:16
Range (%)
87-103
85-99
87-100
Average (%)
94
92
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
91
EDTA plasma (n=5)
86-98
93
Cell culture media (n=5)
90-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.33
15.96
36.82
4.96
15.73
38.14
Standard deviation
0.28
0.82
1.12
0.34
0.83
1.63
C V (%)
5.25
5.14
3.04
6.85
5.28
4.27
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MAC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MAC in samples. No significant cross-reactivity or interference between Human MAC and analogues was observed.
