Human LTF Recombinant Protein (RPPB4062)
- SKU:
- RPPB4062
- Product type:
- Recombinant Protein
- Size:
- 200ug
- Species:
- Human
- Target:
- LTF
- Synonyms:
- Lactotransferrin
- Lactoferrin
- Growth-inhibiting protein 12
- Talalactoferrin
- Source:
- Human breast milk
- Uniprot:
- P02788
Description
| Product Name: | Human LTF Recombinant Protein |
| Product Code: | RPPB4062 |
| Size: | 200µg |
| Species: | Human |
| Target: | LTF |
| Synonyms: | Lactotransferrin, Lactoferrin, Growth-inhibiting protein 12, Talalactoferrin, LTF, GIG12, LF, HLF2, Neutrophil Lactoferrin. |
| Source: | Human breast milk |
| Physical Appearance: | Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | LTF protein filtered (0.4µm) and lyophilized in 0.5 mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4. |
| Solubility: | It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Stability: | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | GRRRSVQWCA VSQPEATKCF QWQRNMRKVR GPPVSCIKRD SPIQCIQAIA ENRADAVTLD GGFIYEAGLA PYKLRPVAAE VYGTERQPRT HYYAVAVVKK GGSFQLNELQ GLKSCHTGLR RTAGWNVPIG TLRPFLNWTG PPEPIEAAVA RFFSASCVPG ADKGQFPNLC RLCAGTGENK CAFSSQEPYF SYSGAFKCLR DGAGDVAFIR ESTVFEDLSD EAERDEYELL CPDNTRKPVD KFKDCHLARV PSHAVVARSV NGKEDAIWNL LRQAQEKFGK DKSPKFQLFG SPSGQKDLLF KDSAIGFSRV PPRIDSGLYL GSGYFTAIQN LRKSEEEVAA RRARVVWCAV GEQELRKCNQ WSGLSEGSVT CSSASTTEDC IALVLKGEAD AMSLDGGYVY TAGKCGLVPV LAENYKSQQS SDPDPNCVDR PVEGYLAVAV VRRSDTSLTW NSVKGKKSCH TAVDRTAGWN IPMGLLFNQT GSCKFDEYFS QSCAPGSDPR SNLCALCIGD EQGENKCVPN SNERYYGYTG AFRCLAENAG DVAFVKDVTV LQNTDGNNNE AWAKDLKLAD FALLCLDGKR KPVTEARSCH LAMAPNHAVV SRMDKVERLK QVLLHQQAKF GRNGSDCPDK FCLFQSETKN LLFNDNTECL ARLHGKTTYE KYLGPQYVAG ITNLKKCSTS PLLEACEFLR K |
Lactoferrin is a glycoprotein that belongs to the transferrin family of iron binding proteins. It is found in human breast milk as well as most epithelial surface secretions including tears, nasogastric, saliva, and bronchial. Lactoferrin binds 2 molecules of iron with very high affinity. Lactoferrin inhibits bacterial growth by withholding iron, its N-terminal region is an antimicrobial peptide. Lactotransferrin acts synergistically with lysozyme to potentiate the activity of both proteins. The multifunctional protein lactoferrin has many physiological possible roles. It is often referred to as an innate defense protein and frequently serves as the first line of defense in protection against pathogens. It has been shown to have the ability to bind iron, it is a natural anti-bacterial, anti-fungal and anti-viral, it is an antioxidant and it also has immunomodulatory properties. It has many beneficial properties, which make it a good candidate for a number of product applications. Considerable research is currently going on to explain the various suggested biological functions of lactoferrin.
The Human Lactoferrin produced from Human breast milk has a molecular mass of 76.165kDa (calculated without glycosylation) containing 691 amino acid residues.
| UniProt Protein Function: | LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter. |
| UniProt Protein Details: | Protein type:Secreted; Protease; EC 3.4.21.-; Secreted, signal peptide Chromosomal Location of Human Ortholog: 3p21.31 Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; nucleus; protein complex; secretory granule; specific granule Molecular Function:heparin binding; iron ion binding; protein binding; protein serine/threonine kinase activator activity Biological Process: activation of NF-kappaB transcription factor; antibacterial humoral response; antifungal humoral response; cell redox homeostasis; cellular protein metabolic process; humoral immune response; innate immune response in mucosa; iron assimilation by chelation and transport; negative regulation of apoptosis; negative regulation of ATPase activity; negative regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of viral genome replication; negative regulation of viral reproduction; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of osteoblast differentiation; positive regulation of osteoblast proliferation; positive regulation of toll-like receptor 4 signaling pathway; regulation of cytokine production; regulation of tumor necrosis factor production; retinal homeostasis |
| NCBI Summary: | This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014] |
| UniProt Code: | P02788 |
| NCBI GenInfo Identifier: | 85700158 |
| NCBI Gene ID: | 4057 |
| NCBI Accession: | P02788.6 |
| UniProt Secondary Accession: | P02788,O00756, Q16780, Q16785, Q16786, Q16789, Q5DSM0 Q8IU92, A8K9U8, B2MV13, B7Z4X2, E7EQH5, |
| UniProt Related Accession: | P02788 |
| Molecular Weight: | 73,161 Da |
| NCBI Full Name: | Lactotransferrin |
| NCBI Synonym Full Names: | lactotransferrin |
| NCBI Official Symbol: | LTF |
| NCBI Official Synonym Symbols: | LF; HLF2; GIG12; HEL110 |
| NCBI Protein Information: | lactotransferrin |
| UniProt Protein Name: | Lactotransferrin |
| UniProt Synonym Protein Names: | Growth-inhibiting protein 12; Talalactoferrin |
| Protein Family: | Lactotransferrin |
| UniProt Gene Name: | LTF |
| UniProt Entry Name: | TRFL_HUMAN |
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