The Human LTF (Lactoferrin) ELISA Kit is specifically designed for the precise measurement of lactoferrin levels in human samples such as serum, plasma, and cell culture supernatants. With a high level of sensitivity and specificity, this kit ensures accurate and consistent results, making it suitable for various research applications.Lactoferrin is a multifunctional protein involved in various biological processes, including immune response modulation, antimicrobial activity, and iron metabolism regulation.
Its levels have been linked to a range of health conditions, including infections, inflammation, and cancer, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.Overall, the Human LTF (Lactoferrin) ELISA Kit is a reliable tool for researchers looking to explore the role of lactoferrin in human health and disease, providing a valuable insight into its potential as a diagnostic and therapeutic target.
Product Name:
Human LTF (Lactoferrin) ELISA Kit
SKU:
HUES03395
Target:
Human LTF (Lactoferrin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LTF/LF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LTF/LF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LTF/LF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LTF/LF. You can calculate the concentration of Human LTF/LF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
86-97
95-109
Average (%)
105
91
100
1:4
Range (%)
93-107
86-102
94-106
Average (%)
100
93
100
1:8
Range (%)
101-115
81-93
93-107
Average (%)
107
87
98
1:16
Range (%)
95-110
86-99
95-112
Average (%)
102
92
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
91
EDTA plasma (n=5)
89-103
95
Cell culture media (n=5)
94-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1
2.1
9.1
1.0
2.2
9.3
Standard deviation
0.1
0.1
0.4
0.1
0.1
0.4
C V (%)
10
4.76
4.4
10.0
4.55
4.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human LTF/LF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LTF/LF in samples. No significant cross-reactivity or interference between Human LTF/LF and analogues was observed.
