The Human LP-A (Lipoprotein (a)) ELISA Kit is a valuable tool for detecting Lipoprotein (a) levels in human serum and plasma samples. With its high sensitivity and specificity, this kit ensures accurate and reproducible results for a variety of research applications.Lipoprotein (a) is a lipoprotein particle that has been linked to an increased risk of cardiovascular disease, making it an important biomarker for studying and understanding heart health.
Elevated levels of Lipoprotein (a) have been associated with a higher risk of heart attacks and strokes, highlighting the significance of monitoring and measuring these levels.This ELISA kit is a valuable resource for researchers and clinicians looking to investigate Lipoprotein (a) levels in various disease states and conditions, offering a reliable and precise method for quantifying this important biomarker.
Product Name:
Human LP-a (Lipoprotein a) ELISA Kit
SKU:
HUES01432
Target:
Human LP-a (Lipoprotein a)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.14 ng/mL
Detection range:
0.23-15 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LP-a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LP-a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LP-a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LP-a. You can calculate the concentration of Human LP-a in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
87-100
90-105
Average (%)
101
92
96
1:4
Range (%)
88-104
79-91
83-93
Average (%)
95
85
88
1:8
Range (%)
92-107
82-96
85-95
Average (%)
98
89
90
1:16
Range (%)
88-99
82-93
86-100
Average (%)
94
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
97
EDTA plasma (n=5)
93-109
100
Cell culture media (n=5)
91-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.78
1.44
6.59
0.74
1.36
6.54
Standard deviation
0.05
0.08
0.22
0.05
0.08
0.32
C V (%)
6.41
5.56
3.34
6.76
5.88
4.89
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human LP-a concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LP-a in samples. No significant cross-reactivity or interference between Human LP-a and analogues was observed.
