The Human LOX (Lysyl Oxidase) ELISA Kit is a highly sensitive and specific assay designed for the precise quantification of LOX levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for various research applications.LOX is a key enzyme involved in the crosslinking of collagen and elastin in the extracellular matrix, playing a crucial role in tissue development and remodeling.
Dysregulation of LOX has been linked to various conditions such as fibrosis, cancer, and cardiovascular diseases, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.With its high sensitivity and specificity, the Human LOX ELISA Kit from Assay Genie is an essential tool for researchers seeking to understand the role of LOX in health and disease, and for developing targeted treatments to address LOX-related pathologies.
Product Name:
Human LOX (Lysyl Oxidase) ELISA Kit
SKU:
HUES01442
Target:
Human LOX (Lysyl Oxidase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LOX. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LOX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LOX, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LOX. You can calculate the concentration of Human LOX in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
98-116
99-114
Average (%)
105
106
106
1:4
Range (%)
86-97
83-94
80-93
Average (%)
92
88
87
1:8
Range (%)
87-101
86-97
82-95
Average (%)
93
92
89
1:16
Range (%)
88-98
80-92
88-99
Average (%)
93
86
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-104
99
EDTA plasma (n=5)
87-100
93
Cell culture media (n=5)
93-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.96
4.41
16.22
2.09
4.47
16.14
Standard deviation
0.11
0.24
0.56
0.12
0.19
0.54
C V (%)
5.61
5.44
3.45
5.74
4.25
3.35
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human LOX concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LOX in samples. No significant cross-reactivity or interference between Human LOX and analogues was observed.
