Human LOX-1 (Lectin Like Oxidized Low Density Lipoprotein Receptor 1) ELISA Kit
The Human LOX-1 (Lectin-like oxidized low-density lipoprotein receptor 1) ELISA Kit is specifically designed for the precise measurement of LOX-1 levels in human samples such as serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and accuracy, ensuring reliable and consistent results for a variety of research purposes.LOX-1 is a key receptor involved in the recognition and uptake of oxidized low-density lipoproteins (LDL), playing a crucial role in the development and progression of various cardiovascular diseases.
Elevated levels of LOX-1 have been linked to atherosclerosis, hypertension, and other cardiovascular conditions, making it an important biomarker for studying and understanding these diseases.With this ELISA kit, researchers can accurately quantify LOX-1 levels in human samples, providing valuable insights into the role of this receptor in cardiovascular health and disease. This kit is an essential tool for studying LOX-1 biology and investigating potential therapeutic strategies for cardiovascular disorders.
Product Name:
Human LOX-1 (Lectin Like Oxidized Low Density Lipoprotein Receptor 1) ELISA Kit
SKU:
HUES02637
Target:
Human LOX-1 (Lectin Like Oxidized Low Density Lipoprotein Receptor 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LOX-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LOX-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LOX-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LOX-1. You can calculate the concentration of Human LOX-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
89-101
91-107
Average (%)
104
94
98
1:4
Range (%)
93-108
85-99
83-93
Average (%)
99
90
88
1:8
Range (%)
91-102
79-92
84-99
Average (%)
96
86
91
1:16
Range (%)
92-107
84-98
87-102
Average (%)
100
91
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
89-101
96
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.43
3.4
0.56
1.31
3.29
Standard deviation
0.04
0.06
0.12
0.03
0.07
0.17
C V (%)
7.55
4.2
3.53
5.36
5.34
5.17
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human LOX-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LOX-1 in samples. No significant cross-reactivity or interference between Human LOX-1 and analogues was observed.
