Human LIPG Recombinant Protein (RPPB1907)
- SKU:
- RPPB1907
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- LIPG
- Synonyms:
- LIPG
- Lipase Endothelial
- EDL
- EL
- Source:
- HEK293 Cells
- Uniprot:
- Q9Y5X9
Description
| Product Name: | Human LIPG Recombinant Protein |
| Product Code: | RPPB1907 |
| Size: | 10µg |
| Species: | Human |
| Target: | LIPG |
| Synonyms: | LIPG, Lipase Endothelial, EDL, EL, Endothelial Cell-Derived Lipase, EC 3.1.1.3, PRO719, Endothelial Lipase, Lipoprotein Lipase H, EC 3.1.1. |
| Source: | HEK293 Cells |
| Physical Appearance: | Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | LIPG was filtered (0.4 µm) and lyophilized from 0.5mg/ml solution in phosphate buffered saline pH 7.5 (PBS), 1% (w/v) Sucrose and 4% (w/v) Mannitol. |
| Solubility: | It is recommended to add 200µl deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. LIPG is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Stability: | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ASSPVPFGPE GRLEDKLHKP KATQTEVKPS VRFNLRTSKD PEHEGCYLSV GHSQPLEDCS FNMTAKTFFI IHGWTMSGIF ENWLHKLVSA LHTREKDANV VVVDWLPLAH QLYTDAVNNT RVVGHSIARM LDWLQEKDDF SLGNVHLIGY SLGAHVAGYA GNFVKGTVGR ITGLDPAGPM FEGADIHKRL SPDDADFVDV LHTYTRSFGL SIGIQMPVGH IDIYPNGGDF QPGCGLNDVL GSIAYGTITE VVKCEHERAV HLFVDSLVNQ DKPSFAFQCT DSNRFKKGIC LSCRKNRCNS IGYNAKKMRN KRNSKMYLKT RAGMPFRVYH YQMKIHVFSY KNMGEIEPTF YVTLYGTNAD SQTLPLEIVE RIEQNATNTF LVYTEEDLGD LLKIQLTWEG ASQSWYNLWK EFRSYLSQPR NPGRELNIRR IRVKSGETQR KLTFCTEDPE NTSISPGREL WFRKCRDGWR MKNETSPTVE LP KLHHHHHH |
Lipase Endothelial (LIPG) has extensive phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. The LIPG protein is considered a member of the TG lipase family through its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. In addition, the LIPG has triglyceride lipase activities. LIPG hydrolyzes HDLs more efficiently than other lipoproteins. LIPG also binds heparin.
LIPG Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (Ser21-Pro500) containing a total of 490 amino acids, having a calculated molecular mass of 55.8kDa. LIPG is fused to a 2 aa N-terminal linker, a 2 aa C-terminal linker and a 6 aa His tag at C-Terminus.
| UniProt Protein Function: | LIPG: Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin. Belongs to the AB hydrolase superfamily. Lipase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Lipid Metabolism - glycerolipid; Phospholipase; Secreted; EC 3.1.1.3; Secreted, signal peptide Chromosomal Location of Human Ortholog: 18q21.1 Cellular Component: Golgi apparatus; extracellular space; cell surface; early endosome Molecular Function:heparin binding; lipoprotein lipase activity; phospholipase A1 activity; phospholipase activity Biological Process: cell proliferation; cholesterol homeostasis; reverse cholesterol transport; phospholipid catabolic process; regulation of lipoprotein metabolic process; lipid metabolic process; positive regulation of cholesterol transport; response to nutrient; phospholipid homeostasis |
| NCBI Summary: | The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9Y5X9 |
| NCBI GenInfo Identifier: | 22001808 |
| NCBI Gene ID: | 9388 |
| NCBI Accession: | Q9Y5X9.1 |
| UniProt Secondary Accession: | Q9Y5X9,Q6P9C8, Q6UW82, B0LPG6, |
| UniProt Related Accession: | Q9Y5X9 |
| Molecular Weight: | 500 |
| NCBI Full Name: | Endothelial lipase |
| NCBI Synonym Full Names: | lipase, endothelial |
| NCBI Official Symbol: | LIPG |
| NCBI Official Synonym Symbols: | EL; EDL; PRO719 |
| NCBI Protein Information: | endothelial lipase; lipoprotein lipase H; endothelial cell-derived lipase |
| UniProt Protein Name: | Endothelial lipase |
| UniProt Synonym Protein Names: | Endothelial cell-derived lipase; EDL; EL |
| Protein Family: | Endothelial lipase |
| UniProt Gene Name: | LIPG |
| UniProt Entry Name: | LIPE_HUMAN |
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