Human LIF Recombinant Protein (RPPB0738)
- SKU:
- RPPB0738
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- LIF
- Synonyms:
- Leukemia Inhibitory Factor
- Differentiation Inhibitory Activity
- Cholinergic Differentiation Factor
- Differentiation-Stimulating Factor
- Source:
- Sf9 Insect cells
- Uniprot:
- P15018
Description
| Product Name: | Human LIF Recombinant Protein |
| Product Code: | RPPB0738 |
| Size: | 10µg |
| Species: | Human |
| Target: | LIF |
| Synonyms: | Leukemia Inhibitory Factor, Differentiation Inhibitory Activity, Cholinergic Differentiation Factor, Differentiation-Stimulating Factor, Hepatocyte-Stimulating Factor III, Differentiation-Inducing Factor, Melanoma-Derived LPL Inhibitor, Human Interleukin In DA Cells, D Factor, HILDA, MLPLI, Emfilermin, DIA, CDF, Leukemia inhibitory factor, LIF, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterilefiltered colorless solution. |
| Formulation: | LIF protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greaterthan 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADPSPLPITP VNATCAIRHP CHNNLMNQIR SQLAQLNGSA NALFILYYTA QGEPFPNNLD KLCGPNVTDF PPFHANGTEK AKLVELYRIV VYLGTSLGNI TRDQKILNPS ALSLHSKLNA TADILRGLLS NVLCRLCSKY HVGHVDVTYG PDTSGKDVFQ KKKLGCQLLG KYKQIIAVLAQAFHHHHHH |
| Biological Activity: | Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 for this effects is less or equal to 0.5 ng/ml. |
Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence.
LIF Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 189 amino acids (23-202a.a.) and having a molecular mass of 20.8kDa (Molecular size on SDS-PAGE will appear at approximately 18-40kDa). LIF is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. |
| NCBI Summary: | The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] |
| UniProt Code: | P15018 |
| NCBI GenInfo Identifier: | 126279 |
| NCBI Gene ID: | 3976 |
| NCBI Accession: | P15018.1 |
| UniProt Secondary Accession: | P15018,Q52LZ2, B2RCW7, B5MC23, |
| UniProt Related Accession: | P15018 |
| Molecular Weight: | 9,563 Da |
| NCBI Full Name: | Leukemia inhibitory factor |
| NCBI Synonym Full Names: | LIF, interleukin 6 family cytokine |
| NCBI Official Symbol: | LIF |
| NCBI Official Synonym Symbols: | CDF; DIA; HILDA; MLPLI |
| NCBI Protein Information: | leukemia inhibitory factor |
| UniProt Protein Name: | Leukemia inhibitory factor |
| UniProt Synonym Protein Names: | Differentiation-stimulating factor; D factor; Melanoma-derived LPL inhibitor; MLPLI; INN: Emfilermin |
| Protein Family: | Leukemia inhibitory factor |
| UniProt Gene Name: | LIF |
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