Human LIF Recombinant Protein (E.coli) (RPES3364)
- SKU:
- RPES3364
- Product type:
- Recombinant Protein
- Host species:
- E.coli
- Reactivity:
- Human
Frequently bought together:
Description
system_update_alt데이터시트
Human LIF Recombinant Protein (E.coli)
Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation; control of stem cell pluripotency; bone and fat metabolism; mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid level. Human LIF is equally active on human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells than human LIF.
| Product Name: | Human LIF Recombinant Protein (E.coli) (RPES3364) |
| Product Code: | RPES3364 |
| Size: | 10µg |
| Species: | Human |
| Expressed Host: | E.coli |
| Synonyms: | Leukemia Inhibitory Factor, LIF, Differentiation-Stimulating Factor, D Factor, Melanoma-Derived LPL Inhibitor, MLPLI, Emfilermin, LIF, HILDA,CDF,DIA |
| Accession: | P15018 |
| Sequence: | Ser23-Phe202 |
| Fusion tag: | |
| Activity: | Measured in a cell proliferation assay using TF?1 human erythroleukemic cells. The ED50 for this effect is 32.1 pg/ml. |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant Human Leukemia Inhibitory Factor is produced by our E.coli expression system and the target gene encoding Ser23-Phe202 is expressed. |
| Purity: | > 95 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 19.7 kDa |
| AP Mol Mass: | 18 kDa |
| Formulation: | Lyophilized from a 0.2 µm filtered solution of 20mM PB; 150mM NaCl; 0.02% Tween 20; pH 7.4. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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