The Human Leukemia Inhibitory Factor (LIF) ELISA Kit is specifically designed for the accurate measurement of LIF levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for various research applications.LIF is a key cytokine known for its role in regulating stem cell differentiation, immune response modulation, and embryo development. Dysregulation of LIF has been linked to various diseases, including leukemia, inflammatory disorders, and infertility.
Therefore, the Human LIF ELISA Kit serves as an indispensable tool for investigating the pathophysiology of these conditions and exploring potential therapeutic interventions.With its user-friendly protocol and reliable performance, the Human LIF ELISA Kit from Assay Genie is a valuable asset for researchers and clinicians seeking to advance their understanding of LIF biology and its implications in human health and disease.
Product Name:
Human LIF (Leukemia Inhibitory Factor) ELISA Kit
SKU:
HUES01380
Target:
Human LIF (Leukemia Inhibitory Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LIF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LIF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LIF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LIF. You can calculate the concentration of Human LIF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
88-98
88-104
Average (%)
100
93
95
1:4
Range (%)
89-102
86-100
86-99
Average (%)
94
91
91
1:8
Range (%)
88-100
86-97
86-101
Average (%)
94
92
93
1:16
Range (%)
87-101
86-101
85-98
Average (%)
93
92
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
93
EDTA plasma (n=5)
91-102
96
Cell culture media (n=5)
92-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.61
298.37
716.65
97.1
285.07
782.41
Standard deviation
6.03
12.14
26.01
5.88
15.17
29.73
C V (%)
5.93
4.07
3.63
6.06
5.32
3.8
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human LIF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LIF in samples. No significant cross-reactivity or interference between Human LIF and analogues was observed.
