Human LGALS8 Recombinant Protein (RPPB0287)
- SKU:
- RPPB0287
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- LGALS8
- Synonyms:
- Galectin-8
- Gal-8
- Po66 carbohydrate-binding protein
- Po66-CBP
- Source:
- Escherichia Coli
- Uniprot:
- O00214
Description
| Product Name: | Human LGALS8 Recombinant Protein |
| Product Code: | RPPB0287 |
| Size: | 10µg |
| Species: | Human |
| Target: | LGALS8 |
| Synonyms: | Galectin-8, Gal-8, Po66 carbohydrate-binding protein, Po66-CBP, Prostate carcinoma tumor antigen 1, PCTA-1, LGALS8. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | LGALS8 was lyophilized from a concentrated (1mg/ml) solution in 20mM PBS, pH 7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized Galectin-8 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized LGALS8 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Galectin-8 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Met-Leu-Ser-Leu |
| Biological Activity: | The ED50 of Galectin-8 as determined by its ability to agglutinate human red blood cells is 0.8~4 µg/ml. |
Galectins are a family of animal lectins with an affinity for beta-galactosides. This family has at least 14 identified members. Galectins share similarities in the CRD (the carbohydrate recognition domain). Galectins are synthesized as cytosolic proteins. Though localized principally in the cytoplasm and lacking a classical signal peptide, galectins can also be stimulated to secretion by non-classical pathways or alternatively targeted to the nucleus. Galectins are involved in modulating cell-cell and cell-matrix interactions. Galectin-8 is a tandem-repeat-type member of the galectin family, consisting of 2 CRDs attached by a linker peptide. Galectin-8 is greatly expressed in lung carcinomas, a number of forms of prostate carcinomas, in addition to other tumor cells. Galectin-8 attaches to a subset of cell surface integrins to modulate ECM-integrin interactions. Once immobilized, Galectin-8 promotes cell adhesion by ligation and clustering of cell surface integrin receptors. On the other hand, as a soluble ligand, Galectin-8 can inhibit cell adhesion.
Galectin-8 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 317 amino acids and having a molecular mass of 35.8kDa.The LGALS8 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | galectin-8: 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell adhesion Chromosomal Location of Human Ortholog: 1q43 Cellular Component: cytosol; extracellular space; membrane Molecular Function:carbohydrate binding; protein binding |
| NCBI Summary: | This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O00214 |
| NCBI GenInfo Identifier: | 292495094 |
| NCBI Gene ID: | 3964 |
| NCBI Accession: | O00214.4 |
| UniProt Secondary Accession: | O00214,O15215, Q5T3P5, Q5T3Q4, Q8TEV1, Q96B92, Q9BXC8 Q9H584, Q9H585, Q9UEZ6, Q9UP32, Q9UP33, |
| UniProt Related Accession: | O00214 |
| Molecular Weight: | 40,397 Da |
| NCBI Full Name: | Galectin-8 |
| NCBI Synonym Full Names: | galectin 8 |
| NCBI Official Symbol: | LGALS8 |
| NCBI Official Synonym Symbols: | Gal-8; PCTA1; PCTA-1; Po66-CBP |
| NCBI Protein Information: | galectin-8 |
| UniProt Protein Name: | Galectin-8 |
| UniProt Synonym Protein Names: | Po66 carbohydrate-binding protein; Po66-CBP; Prostate carcinoma tumor antigen 1; PCTA-1 |
| Protein Family: | Galectin |
| UniProt Gene Name: | LGALS8 |
| UniProt Entry Name: | LEG8_HUMAN |
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