CD53 is a member of the transmembrane 4 superfamily, also called the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. These proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD53 is a cell surface glycoprotein that is known to complex with integrins. Familial deficiency of CD53 gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. CD53 contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
1. Measured by its binding ability in a functional ELISA. Immobilized human CD2-His at 10 µg/ml (100 ?l/well) can bind human CD58-Fc, The EC50 of human CD58-Fc is 0.04-0.1 µg/ml.2. Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus CD2-His at 10 µg/ml (100 ?l/well) can bind human CD58-Fc, The EC50 of human CD58-Fc is 0.04-0.10 µg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human CD58 (Q9BRW0) (Met1-Arg215) was expressed with the Fc region of human IgG1 at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
48.5 kDa
AP Mol Mass:
68 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
