The Human LEP (Leptin) CLIA Kit is a cutting-edge tool for the precise measurement of Leptin levels in human samples, including serum, plasma, and cell culture supernatants. This advanced kit offers outstanding sensitivity and specificity, allowing for accurate and reliable results that are perfect for a variety of research applications.Leptin is a key hormone that regulates appetite and energy expenditure, making it a crucial player in metabolic processes and obesity. Imbalances in Leptin levels are associated with various health conditions, including diabetes, cardiovascular diseases, and obesity-related disorders.
Therefore, monitoring Leptin levels is important for understanding these conditions and developing effective treatments.The Human LEP (Leptin) CLIA Kit from Assay Genie provides researchers with a powerful tool to study Leptin and its role in human health and disease. With its high-quality components and user-friendly design, this kit is a valuable asset for any laboratory looking to delve into the fascinating world of Leptin biology.
Product Name:
Human LEP (Leptin) CLIA Kit
SKU:
HUES00108
Target:
Human LEP (Leptin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LEP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LEP. You can calculate the concentration of Human LEP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-106
87-102
99-115
Average (%)
97
94
106
1:4
Range (%)
92-104
99-113
98-114
Average (%)
98
105
104
1:8
Range (%)
100-114
93-107
93-104
Average (%)
107
101
99
1:16
Range (%)
88-101
96-111
88-100
Average (%)
95
102
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-106
101
EDTA plasma (n=5)
97-111
104
Cell culture media (n=5)
87-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.9
288.04
860.68
97.37
310.44
819.04
Standard deviation
9.24
21.92
71.87
8.68
22.63
64.29
C V (%)
8.56
7.61
8.35
8.91
7.29
7.85
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LEP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LEP in samples. No significant cross-reactivity or interference between Human LEP and analogues was observed.
