Human LDLR (Low Density Lipoprotein Receptor) ELISA Kit
The Human LDLR (Low Density Lipoprotein Receptor) ELISA Kit is a high-quality assay designed for the precise measurement of LDLR levels in human serum and plasma samples. This kit provides accurate and reliable results, with high sensitivity and specificity, making it ideal for a variety of research applications.The Low Density Lipoprotein Receptor (LDLR) is a critical protein involved in the regulation of cholesterol metabolism and homeostasis. Dysfunction of the LDLR has been linked to a variety of cardiovascular diseases, including atherosclerosis and familial hypercholesterolemia.
Monitoring LDLR levels can provide valuable insights into these conditions and aid in the development of potential therapeutic strategies.Overall, the Human LDLR ELISA Kit offers researchers a valuable tool for studying LDLR biology and its implications in disease. Its easy-to-use format and robust performance make it a valuable addition to any laboratory conducting research in the field of lipid metabolism and cardiovascular health.
Product Name:
Human LDLR (Low Density Lipoprotein Receptor) ELISA Kit
SKU:
HUES02282
Target:
Human LDLR (Low Density Lipoprotein Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LDLR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LDLR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LDLR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LDLR. You can calculate the concentration of Human LDLR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
88-102
90-102
Average (%)
98
94
95
1:4
Range (%)
87-97
84-96
82-96
Average (%)
92
91
88
1:8
Range (%)
91-104
87-98
87-101
Average (%)
98
92
94
1:16
Range (%)
94-108
85-99
84-97
Average (%)
100
92
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-108
98
EDTA plasma (n=5)
87-103
94
Cell culture media (n=5)
94-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.01
1.7
7.95
0.95
1.81
8.59
Standard deviation
0.05
0.09
0.38
0.05
0.08
0.37
C V (%)
4.95
5.29
4.78
5.26
4.42
4.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human LDLR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LDLR in samples. No significant cross-reactivity or interference between Human LDLR and analogues was observed.
