Human LDHB Recombinant Protein (RPPB1900)
- SKU:
- RPPB1900
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- LDHB
- Synonyms:
- Lactate Dehydrogenase B
- Renal Carcinoma AntigenNY-REN-46
- LDH Heart Subunit
- EC 11127
- Source:
- Escherichia Coli
- Uniprot:
- P07195
Frequently bought together:
Description
| Product Name: | Human LDHB Recombinant Protein |
| Product Code: | RPPB1900 |
| Size: | 10µg |
| Species: | Human |
| Target: | LDHB |
| Synonyms: | Lactate Dehydrogenase B, Renal Carcinoma AntigenNY-REN-46, LDH Heart Subunit, EC 1.1.1.27, LDH-B, LDH-H, Epididymis SecretoryProtein Li 281, Testicular Secretory Protein Li 25, Lactate Dehydrogenase HChain, HEL-S-281, EC 1.1.1 , LDHBD, TRG-5. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The LDHB solution (1mg/ml) contains 20mM Tris-HCl (pH 8.0), 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greaterthan 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MATLKEKLIAPVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVADKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWKLSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMGTDNDSENWKE VHKMVVESAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFLSLPCILNARG LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL |
| Biological Activity: | Specific activity is >300 units/mg, in which 1 unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per min at pH 7.5 at 37°. |
LDHB is part of the lactate dehydrogenase family. LDHB is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. LDHB catalyzes the oxidation of hydroxybutyrate, and frequently called Hydroxybutyrate Dehydrogenase (HBD). The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors.
LDHB Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 334 amino acids (1-334) and having a molecular mass of 36.6kDa.
| NCBI Summary: | This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. [provided by RefSeq, Feb 2016] |
| UniProt Code: | P07195 |
| NCBI GenInfo Identifier: | 126041 |
| NCBI Gene ID: | 3945 |
| NCBI Accession: | P07195.2 |
| UniProt Related Accession: | P07195 |
| Molecular Weight: | ~ 37kDa |
| NCBI Full Name: | L-lactate dehydrogenase B chain |
| NCBI Synonym Full Names: | lactate dehydrogenase B |
| NCBI Official Symbol: | LDHB |
| NCBI Official Synonym Symbols: | LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281 |
| NCBI Protein Information: | L-lactate dehydrogenase B chain |
| UniProt Protein Name: | L-lactate dehydrogenase B chain |
| UniProt Synonym Protein Names: | LDH heart subunit; LDH-H; Renal carcinoma antigen NY-REN-46 |
| Protein Family: | L-lactate dehydrogenase |
| UniProt Gene Name: | LDHB |
| UniProt Entry Name: | LDHB_HUMAN |
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