Human LDHB Recombinant Protein (RPPB1899)

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SKU:
RPPB1899
Product type:
Recombinant Protein
Size:
25ug
Species:
Human
Target:
LDHB
Synonyms:
LDH-H
Synonyms:
TRG-5
Synonyms:
L-lactate dehydrogenase B chain
Synonyms:
LDH-B
Source:
Escherichia Coli
Uniprot:
P07195
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Description

Product Name:Human LDHB Recombinant Protein
Product Code:RPPB1899
Size:25µg
Species:Human
Target:LDHB
Synonyms:LDH-H, TRG-5, L-lactate dehydrogenase B chain, LDH-B, EC=1.1.1.27, Renal carcinoma antigen NY-REN-46, LDHB.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:LDHB Human solution containing 20mM Tris-HCl pH-8, 1mM DTT, & 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MATLKEKLIA PVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL
Biological Activity:Specific activity is > 6 units/mg. in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37°C.

LDHB is part of the lactate dehydrogenase family. LDHB is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. LDHB catalyzes the oxidation of hydroxybutyrate, and frequently called Hydroxybutyrate Dehydrogenase (HBD). The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors.

LDHB Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 354 amino acids (1-334 a.a.) and having a molecular mass of 38.8 kDa. The LDHB is fused to a 20 amino acids His-Tag at N-terminus and purified by proprietary chromatographic techniques.

NCBI Summary:This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. [provided by RefSeq, Feb 2016]
UniProt Code:P07195
NCBI GenInfo Identifier:126041
NCBI Gene ID:3945
NCBI Accession:P07195.2
UniProt Related Accession:P07195
Molecular Weight:~ 37kDa
NCBI Full Name:L-lactate dehydrogenase B chain
NCBI Synonym Full Names:lactate dehydrogenase B
NCBI Official Symbol:LDHB  
NCBI Official Synonym Symbols:LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281  
NCBI Protein Information:L-lactate dehydrogenase B chain
UniProt Protein Name:L-lactate dehydrogenase B chain
UniProt Synonym Protein Names:LDH heart subunit; LDH-H; Renal carcinoma antigen NY-REN-46
Protein Family:L-lactate dehydrogenase
UniProt Gene Name:LDHB  
UniProt Entry Name:LDHB_HUMAN
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