The Human LDHA (Lactate Dehydrogenase A) ELISA Kit is a powerful tool for the precise measurement of LDHA levels in human samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for a variety of research investigations.LDHA is an enzyme that plays a key role in cellular metabolism, specifically in the conversion of lactate to pyruvate. Dysregulation of LDHA has been linked to various diseases, including cancer and metabolic disorders, highlighting its importance as a potential biomarker for disease diagnosis and treatment monitoring.
By utilizing the Human LDHA ELISA Kit, researchers can gain valuable insights into the role of LDHA in disease pathogenesis and progression, ultimately facilitating the development of novel therapeutic strategies. Trust in the precision and reliability of this kit to advance your research endeavors and uncover new discoveries in the field of biomedical science.
Product Name:
Human LDHA (Lactate Dehydrogenase A) ELISA Kit
SKU:
HUES01740
Target:
Human LDHA (Lactate Dehydrogenase A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LDHA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LDHA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LDHA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LDHA. You can calculate the concentration of Human LDHA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
89-103
94-108
Average (%)
94
95
99
1:4
Range (%)
99-116
87-100
92-106
Average (%)
106
92
98
1:8
Range (%)
100-116
85-96
95-110
Average (%)
108
90
103
1:16
Range (%)
97-109
84-97
90-106
Average (%)
102
90
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
101
EDTA plasma (n=5)
86-97
92
Cell culture media (n=5)
91-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.3
7.5
20.9
2.2
8.2
21.3
Standard deviation
0.1
0.3
0.9
0.1
0.4
1.1
C V (%)
4.35
4.0
4.31
4.55
4.88
5.16
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human LDHA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LDHA in samples. No significant cross-reactivity or interference between Human LDHA and analogues was observed.
