Lysosomal Associated Membrane Protein 2 (LAMP2) is a major component of lysosomal membranes. LAMP2 is a transmembrane glycoprotein about 110kDa. Mature human LAMP2 consists of a 347 amino acid (aa) intralumenal domain, a 24 aa transmembrane segment, and a 35 aa cytoplasmic tail . The lumenal domain is organized into two heavily N-glycosylated regions. Alternate splicing generates a human LAMP2 isoform (LAMP2B) with a substituted juxtamembrane lumenal region, cytoplasmic tail and transmenmbrane segment.LAMP2 itself can cleavage lysosomal luminal domain and degradation lysosomal. In the help of chaperone HSC73,LAMP2 mediates the lysosomal uptake in complex with cargo proteins and is required for the lysosomal destruction of autophagic vacuoles.
Product Name:
Human LAMP2/CD107b Recombinant Protein (RPES3183)
Product Code:
RPES3183
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Lysosome-Associated Membrane Glycoprotein 2, LAMP-2, Lysosome-Associated Membrane Protein 2, CD107 Antigen-Like Family Member B, CD107b, LAMP2
Accession:
P13473
Sequence:
Leu29-Ile375
Fusion tag:
C-6His
Activity:
Immobilized Human LGALS3(Cat: PKSH032474) at 1.5µg/ml(100 ?l/well) can bind Human LAMP2-His. The ED50 of Human LAMP2-His is 12.73 ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Lysosome-Associated Membrane Glycoprotein 2 is produced by our Mammalian expression system and the target gene encoding Leu29-Ile375 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
33.9 kDa
AP Mol Mass:
78 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
