Human Kruppel Like Factor 2, Lung (KLF2) ELISA Kit
The Human Kruppel-Like Factor 2 (KLF2) ELISA Kit is a powerful tool for the accurate measurement of KLF2 levels in human lung tissue samples, serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications related to lung health.KLF2 is a key transcription factor that plays a crucial role in regulating gene expression in lung cells, impacting important processes such as inflammation, cell differentiation, and lung development.
Dysregulation of KLF2 has been implicated in various respiratory diseases, including asthma, chronic obstructive pulmonary disease (COPD), and lung cancer, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.With its reliable performance and user-friendly design, the Human KLF2 ELISA Kit is an essential tool for researchers and healthcare professionals seeking to deepen their understanding of lung biology and disease mechanisms.
Product Name:
Human Kruppel Like Factor 2, Lung (KLF2) ELISA Kit
Product Code:
HUDL01619
Size:
96 Assays
Target:
KLF2
Synonyms:
LKLF, Lung krueppel-like factor
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
