Human KMT5A Recombinant Protein (RPPB1878)

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SKU:
RPPB1878
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
KMT5A
Synonyms:
KMT5A
Synonyms:
PR-Set7
Synonyms:
SET07
Synonyms:
SET8
Source:
Sf9 Insect cells
Uniprot:
Q9NQR1
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Description

Product Name:Human KMT5A Recombinant Protein
Product Code:RPPB1878
Size:10µg
Species:Human
Target:KMT5A
Synonyms:KMT5A, PR-Set7, SET07, SET8, SETD8, H4-K20-HMTase KMT5A.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered colorless solution.
Formulation:KMT5A protein solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 5mM DTT, 0.2M NaCl, 1mM EDTA and 50% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MKAELQSEER KRIDELIESG KEEGMKIDLI DGKGRGVIAT KQFSRGDFVV EYHGDLIEIT DAKKREALYA QDPSTGCYMY YFQYLSKTYC VDATRETNRL GRLINHSKCG NCQTKLHDID GVPHLILIAS RDIAAGEELL YDYGDRSKAS IEAHPWLKHH HHHHH

Lysine Methyltransferase 5A (KMT5A) is an enzyme which catalyzes both histones and non-histone proteins. KMT5A contributes to the maintenance of proper higher-order structure of DNA during mitosis. KMT5A takes part in cell-cycle-dependent transcriptional silencing and mitotic regulation in metazoans. KMT5A plays a role as a barrier to prevent cellular senescence through chromatinmediated regulation of senescence-associated metabolic remodeling. KMT5A mediates monomethylation of p53/TP53 at 'Lys-382', which leads to repress p53/TP53-target genes. The loss of KMT5A simultaneously stimulate nucleolar function and retinoblastoma protein-mediated mitochondrial metabolism.

KMT5A produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 165 amino acids (195-352 a.a.) and having a molecular mass of 18.9kDa (Migrates at 18-28 kDa on SDS-PAGE under reducing conditions).KMT5A is expressed with a 7 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:SETD8: a protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Methylates K20 of histone H4 (H4K20me1), a specific tag for epigenetic transcriptional repression. SETD8 protein and H4K20me1 levels are cell cycle regulated, both increasing in S phase and peaking at G2/M phase. Interacts with the PCNA protein, associates with sites of active DNA synthesis and is required for DNA replication and genome stability during S phase. Inhibition of SET8 using shRNA results in arrest of replication forks, induction of double-stranded DNA breaks and a Chk1-mediated cell-cycle arrest in S and G2/M phases of the cell cycle. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Inhibition of SET8 using shRNA results in arrest of replication forks, induction of double-stranded DNA breaks and a Chk1-mediated cell-cycle arrest in S and G2/M phases of the cell cycle. Nucleosomes are preferred as substrate compared to free histones. Methylates p53K382, leading to repression of the pro-apoptotic and checkpoint activation functions of p53. SET8 expression levels decrease in response to DNA damage, allowing p53 to activate checkpoints and/or apoptosis. Both the methylation of histone H4K20 and p53K382 appear to be important for the functions of SET8 in S phase. Belongs to the histone-lysine methyltransferase family. PR/SET subfamily. Two isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Methyltransferase, protein lysine; Methyltransferase; EC 2.1.1.43; Amino Acid Metabolism - lysine degradation

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: nucleoplasm; nucleolus; chromosome; nucleus

Molecular Function:protein binding; p53 binding; protein-lysine N-methyltransferase activity; transcription corepressor activity; histone-lysine N-methyltransferase activity

Biological Process: mitosis; transcription, DNA-dependent; cell division; peptidyl-lysine mono-methylation; negative regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; negative regulation of transcription, DNA-dependent; regulation of DNA damage response, signal transduction by p53 class mediator

UniProt Code:Q9NQR1
NCBI GenInfo Identifier:25091219
NCBI Gene ID:387893
NCBI Accession:Q9NQR1.3
UniProt Secondary Accession:Q9NQR1,Q86W83, Q8TD09, A8K9D0,
UniProt Related Accession:Q9NQR1
Molecular Weight:393
NCBI Full Name:N-lysine methyltransferase SETD8
NCBI Synonym Full Names:SET domain containing (lysine methyltransferase) 8
NCBI Official Symbol:SETD8  
NCBI Official Synonym Symbols:SET8; KMT5A; SET07; PR-Set7  
NCBI Protein Information:N-lysine methyltransferase SETD8; PR/SET07; H4-K20-HMTase SETD8; lysine N-methyltransferase 5A; SET domain-containing protein 8; PR/SET domain containing protein 8; PR/SET domain-containing protein 07; histone-lysine N-methyltransferase SETD8; H4-K20-spec
UniProt Protein Name:N-lysine methyltransferase SETD8
UniProt Synonym Protein Names:H4-K20-HMTase SETD8; Histone-lysine N-methyltransferase SETD8 (EC:2.1.1.43); Lysine N-methyltransferase 5A; PR/SET domain-containing protein 07; PR-Set7; PR/SET07; SET domain-containing protein 8
Protein Family:N-lysine methyltransferase
UniProt Gene Name:SETD8  
UniProt Entry Name:SETD8_HUMAN
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