Human KLK1 Recombinant Protein (RPPB1859)
- SKU:
- RPPB1859
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- KLK1
- Synonyms:
- KLK1
- KLK-1
- HK1
- HK-1
- Source:
- Escherichia Coli
- Uniprot:
- P06870
Description
| Product Name: | Human KLK1 Recombinant Protein |
| Product Code: | RPPB1859 |
| Size: | 10µg |
| Species: | Human |
| Target: | KLK1 |
| Synonyms: | KLK1, KLK-1, HK1, HK-1, KLKR, KLK6, Tissue Kallikrein, hKLK1, EC 3.4.21.35, Kidney/pancreas/salivary gland kallikrein, Kallikrein-1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The KLK1 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MIVGGWECEQ HSQPWQAALY HFSTFQCGGI LVHRQWVLTA AHCISDNYQL WLGRHNLFDD ENTAQFVHVS ESFPHPGFNM SLLENHTRQA DEDYSHDLML LRLTEPADTI TDAVKVVELP TQEPEVGSTC LASGWGSIEP ENFSFPDDLQ CVDLKILPND ECKKVHVQKV TDFMLCVGHL EGGKDTCVGD SGGPLMCDGV LQGVTSWGYV PCGTPNKPSV AVRVLSYVKW IEDTIAENS |
Kallikreins are serine protease enzymes having various physiological functions. Kallikreins are implicated in carcinogenesis and have potenital as novel cancer disease biomarkers. KLK1 is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. KLK1 is functionally conserved in its ability to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen.Human Kallikrein-1, also called as Kallidinogenase, Kininogenase or Kininogenin, is an active protein enzyme present in saliva, pancreatic juices, and urine that catalyzes the proteolysis of bradykininogen to bradykinin. Kallikrein-1, which derived from human or porcine, have been used as drugs for a long time, they are mainly used in the treatment f light to medium hypertension and occlusion of cerebral and surrounding blood vessels. KLK1 demonstrates both trypsin- and chymotrypsin-like selectivities with Tyr/Arg preferred at site P1, Ser/Arg strongly preferred at P1', and Phe/Leu at P2. rs5517 in the KLK1 gene is considerably connected with hypertension in a Chinese Han population. KLK1 is expressed de novo in endothelial cells and mediates relaxation of human umbilical veins. The K allele of KLK1 promoter and TT genotype of TGF-beta1 are a genetic KLK1 -130 GN and -128 G-C, and the defenselessness factor contributing to progressive renal descent in Taiwanese primary vesicoureteric reflux children. Induction of KLK1 in carotid arteriosclerosis doesn’t lead to kallikrein-kinins pathway activation. Transgenic rats expressing KLK1 have impaired renal response to acute volume expansion Endothelial cells synthesize and release active form of KLK1on the surface which is important function in maintenance of circulation homeostasis. KLK1 participates in epidermal desquamation through cleavage of desmoglein 1 and regulation by lympho-epithelial Kazal-type-related inhibitor (LEKTI).
Kallikrein-1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 259 amino acids (25-262) and having a molecular mass of 28.7kDa.KLK1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | KLK1: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Belongs to the peptidase S1 family. Kallikrein subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Protease; EC 3.4.21.35 Chromosomal Location of Human Ortholog: 19q13.3 Cellular Component: nucleus Molecular Function:serine-type endopeptidase activity Biological Process: proteolysis Disease: Kallikrein, Decreased Urinary Activity Of |
| NCBI Summary: | Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P06870 |
| NCBI GenInfo Identifier: | 269849612 |
| NCBI Gene ID: | 3816 |
| NCBI Accession: | P06870.2 |
| UniProt Secondary Accession: | P06870,Q66US9, Q86U61, Q8TCV8, Q9BS53, Q9NQU4, Q9UD19 Q9UMJ1, |
| UniProt Related Accession: | P06870 |
| Molecular Weight: | 23,835 Da |
| NCBI Full Name: | Kallikrein-1 |
| NCBI Synonym Full Names: | kallikrein 1 |
| NCBI Official Symbol: | KLK1 |
| NCBI Official Synonym Symbols: | hK1; KLKR; Klk6 |
| NCBI Protein Information: | kallikrein-1; tissue kallikrein; glandular kallikrein 1; kallikrein serine protease 1; kallikrein 1, renal/pancreas/salivary; kidney/pancreas/salivary gland kallikrein |
| UniProt Protein Name: | Kallikrein-1 |
| UniProt Synonym Protein Names: | Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein |
| Protein Family: | Kallikrein 1-related peptidase |
| UniProt Gene Name: | KLK1 |
| UniProt Entry Name: | KLK1_HUMAN |
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