The Human Ki-67 Protein ELISA Kit is specifically designed for the precise measurement of Ki-67 protein levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it perfect for a variety of research purposes.Ki-67 protein is a well-known marker for cell proliferation, making it a key player in processes such as cancer development and tumor growth. By detecting and quantifying Ki-67 levels, researchers can gain valuable insights into cell proliferation activity, cancer progression, and potential therapeutic targets.
This ELISA kit provides researchers with a reliable tool for studying the role of Ki-67 protein in various diseases and conditions, ultimately aiding in the development of novel treatments and therapies. Dive into the world of cell proliferation and cancer research with the Human Ki-67 Protein ELISA Kit.
Product Name:
Human Ki67P (Ki-67 Protein) ELISA Kit
SKU:
HUES03433
Target:
Human Ki67P (Ki-67 Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Ki67P. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Ki67P and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Ki67P, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Ki67P. You can calculate the concentration of Human Ki67P in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
97-110
91-102
Average (%)
95
104
97
1:4
Range (%)
89-101
83-98
89-100
Average (%)
94
89
95
1:8
Range (%)
87-102
83-98
87-99
Average (%)
94
89
94
1:16
Range (%)
86-99
84-96
87-100
Average (%)
92
91
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
96
EDTA plasma (n=5)
95-109
102
Cell culture media (n=5)
91-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.92
3.1
9.4
0.83
3.26
8.87
Standard deviation
0.05
0.13
0.49
0.04
0.17
0.38
C V (%)
5.43
4.19
5.21
4.82
5.21
4.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Ki67P concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Ki67P in samples. No significant cross-reactivity or interference between Human Ki67P and analogues was observed.
