Tissue kallikrein 13 (hK13); also known as KLK-L4 (kallikrein-like gene 4); is a member of the human tissue kallikrein family of serine proteases having diverse physiological functions in many tissues. The KLK13 gene resides on chromosome 19q13.3-4 along with other 14 members in a gene cluster and shares a high degree of homology. KLK13 is a trypsin-like; secreted serine protease expressed specifically in the testicular tissue including prostate; salivary gland; breast; and testis. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and may play a role in metastasis. KLK13 may be involved in the pathogenesis and/or progression of breast and ovary cancers; and is regarded as a novel cancer biomarker. In addition; KLK13 interacts and forms complexes with several serum protease inhibitors; such as alpha2-macroglobulin; and its expression is regulated by steroid hormones.
Product Name:
Human Kallikrein 13/KLK13 Recombinant Protein (RPES0484)
Product Code:
RPES0484
Size:
10µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Kallikrein-13, Kallikrein-Like Protein 4, KLK-L4, KLK13, KLKL4
Accession:
NP_056411.1
Sequence:
Met 1-Ile 262
Fusion tag:
C-His
Activity:
Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (R&D Systems; Catalog # ES011). The specific activity is >200 pmoles/min/µg.(Activation description: The proenzyme needs to be activated by Lysyl-Endopeptidase for an activated form)
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the pro-form of human KLK13 (NP_056411.1) (Met 1-Ile 262) was expressed with a C-terminal polyhistidine tag.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
28.4 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
