The Human ITLN1 (Intelectin-1/Omentin) ELISA Kit is a specialized assay designed for the precise measurement of Intelectin-1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Intelectin-1, also known as Omentin, is a protein with important roles in metabolic regulation, inflammation, and immune response. It has been implicated in various diseases such as obesity, type 2 diabetes, and cardiovascular disorders, making it a valuable marker for understanding these conditions and potential therapeutic strategies.
With its advanced technology and reliable performance, the Human ITLN1 ELISA Kit is an essential tool for researchers seeking to investigate the role of Intelectin-1 in health and disease. Order yours now to advance your research efforts in the field of metabolic and inflammatory disorders.
Product Name:
Human ITLN1 (Intelectin 1/Omentin) ELISA Kit
SKU:
HUES02956
Target:
Human ITLN1 (Intelectin 1/Omentin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ITLN1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ITLN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ITLN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ITLN1. You can calculate the concentration of Human ITLN1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-111
93-111
88-99
Average (%)
102
101
93
1:4
Range (%)
86-98
82-95
89-103
Average (%)
93
87
95
1:8
Range (%)
91-103
81-94
83-96
Average (%)
96
86
88
1:16
Range (%)
92-105
86-102
85-98
Average (%)
98
93
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-110
101
EDTA plasma (n=5)
89-105
96
Cell culture media (n=5)
84-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.98
6.33
16.56
1.83
6.51
17.0
Standard deviation
0.12
0.27
0.67
0.12
0.3
0.53
C V (%)
6.06
4.27
4.05
6.56
4.61
3.12
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ITLN1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ITLN1 in samples. No significant cross-reactivity or interference between Human ITLN1 and analogues was observed.
