Human ISG15 Recombinant Protein (RPPB3757)
- SKU:
- RPPB3757
- Product type:
- Recombinant Protein
- Size:
- 50ug
- Species:
- Human
- Target:
- ISG15
- Synonyms:
- ISG15
- G1P2
- IFI15
- UCRP
- Source:
- Escherichia Coli
- Uniprot:
- P05161
Description
| Product Name: | Human ISG15 Recombinant Protein |
| Product Code: | RPPB3757 |
| Size: | 50µg |
| Species: | Human |
| Target: | ISG15 |
| Synonyms: | ISG15, G1P2, IFI15, UCRP, ISG15 Ubiquitin-like modifier. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The ISG15 solution contains 20mM Tris-HCl pH-8, 100mM NaCl and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 98.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAKG SMGWDLTVKM LAGNEFQVSL SSSMSVSELK AQITQKIGVH AFQQRLAVHP SGVALQDRVP LASQGLGPGS TVLLVVDKCD EPLNILVRNN KGRSSTYEVR LTQTVAHLKQ QVSGLEGVQD DLFWLTFEGK PLEDQLPLGE YGLKPLSTVF MNLRLRGG |
ISG15 ubiquitin-like Modifier is conjugated to the intracellular target proteins after IFN-alpha or IFN-beta stimulation. ISG15 enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the ub conjugation pathway at the level of a specific E2 enzyme. ISG15 protein targets include STAT1, SERPINA 3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MXA, and RIG-1. Deconjugated by USP18/UBP43 shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. ISG15 serves as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. ISG15 plays a role in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing ifn-gamma secretion by monocytes and macrophages. ISG15 displays antiviral activity during viral infections. ISG15 in response to IFN-TAU secreted by the conceptus, ligates to and regulate proteins involved in the release of prostaglandin F2-alpha and thus prevent lysis of the corpus luteum and maintain the pregnancy. ISG15 shares several common properties with other ubiquitin-like molecules, but its activity is strongly regulated by precise signaling pathways that have a role in innate immunity.
ISG15 Human Recombinant fused to N-terminal Calmudulin Tag (151 a.a.) produced in E.Coli is a single, non-glycosylated polypeptide chain containing a total of 308 amino acids (157 a.a. without tag) and having a molecular mass of 34 kDa.
| UniProt Protein Function: | ISG15: Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme) (Probable). Interaction with influenza B NS1 protein inhibits this conjugation. By type I interferons. Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons. |
| UniProt Protein Details: | Protein type:Ubiquitin-like modifier Chromosomal Location of Human Ortholog: 1p36.33 Cellular Component: cytosol; extracellular region; nucleoplasm Molecular Function:protein binding Biological Process: bypass DNA synthesis; defense response to bacterium; defense response to virus; ISG15-protein conjugation; negative regulation of interferon type I production; negative regulation of protein ubiquitination; negative regulation of viral genome replication; positive regulation of bone mineralization; regulation of interferon-gamma production Disease: Immunodeficiency 38 |
| NCBI Summary: | The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012] |
| UniProt Code: | P05161 |
| NCBI GenInfo Identifier: | 52001470 |
| NCBI Gene ID: | 9636 |
| NCBI Accession: | P05161.5 |
| UniProt Secondary Accession: | P05161,Q5SVA4, Q7Z2G2, Q96GF0, |
| UniProt Related Accession: | P05161 |
| Molecular Weight: | 17,888 Da |
| NCBI Full Name: | Ubiquitin-like protein ISG15 |
| NCBI Synonym Full Names: | ISG15 ubiquitin-like modifier |
| NCBI Official Symbol: | ISG15 |
| NCBI Official Synonym Symbols: | G1P2; IP17; UCRP; IFI15; IMD38; hUCRP |
| NCBI Protein Information: | ubiquitin-like protein ISG15 |
| UniProt Protein Name: | Ubiquitin-like protein ISG15 |
| UniProt Synonym Protein Names: | Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; IP17; Ubiquitin cross-reactive protein; hUCRP |
| Protein Family: | Ubiquitin-like protein |
| UniProt Gene Name: | ISG15 |
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