Human IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) ELISA Kit
The Human IP-10 (CXCL10) Interferon Gamma-Induced Protein 10kDa ELISA Kit is a cutting-edge tool designed for the precise measurement of IP-10 levels in human serum, plasma, and cell culture supernatants. This innovative kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it the perfect choice for a diverse array of research endeavors.IP-10, also known as CXCL10, is a critical chemokine involved in the immune response, particularly in the recruitment of T cells to sites of inflammation.
It is induced by interferon-gamma and plays a key role in the pathogenesis of various diseases, including autoimmune disorders and infectious diseases. Monitoring IP-10 levels can provide valuable insights into immune responses and disease progression, making it a valuable biomarker for research and potential therapeutic interventions.
Product Name:
Human IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) ELISA Kit
SKU:
HUES01337
Target:
Human IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IP-10/CXCL10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IP-10/CXCL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IP-10/CXCL10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IP-10/CXCL10. You can calculate the concentration of Human IP-10/CXCL10 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
99-114
90-103
Average (%)
92
104
95
1:4
Range (%)
92-107
86-101
97-110
Average (%)
100
93
103
1:8
Range (%)
96-108
86-100
97-112
Average (%)
103
93
104
1:16
Range (%)
94-111
87-100
92-103
Average (%)
101
92
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
102
EDTA plasma (n=5)
93-111
101
Cell culture media (n=5)
85-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.5
78.8
207.7
25.5
80.2
211.7
Standard deviation
1.5
4.6
11.4
1.7
4.4
11.0
C V (%)
5.66
5.84
5.49
6.67
5.49
5.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IP-10/CXCL10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IP-10/CXCL10 in samples. No significant cross-reactivity or interference between Human IP-10/CXCL10 and analogues was observed.
