IL17; also known as IL17a; is a cytokine belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation; differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes; macrophages; dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members; IL-17/IL-17A; IL-17B; IL-17C; IL-17D; IL-17E/IL-25; and IL-17F; which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2); as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis; psoriasis and multiple sclerosis.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Human Interleukin7A/IL7A Recombinant Protein (RPES0391)
Measured by its binding ability in a functional ELISA. 1.Immobilized human His-IL17A at 10µg/mL (100?L/well) can bind biotinylated human IL17Ra-His; the EC50 of biotinylated human IL17Ra-His is 0.03-0.2µg/mL.2.Measured by its ability to induce IL-6 secretion by HFF human foreskin fibroblast cells. The EC50 for this effect is typically 5-25 ng/ml.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding human IL17A (Q16552) (Gly24-Ala155) was expressed with a polyhistidine tag at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
17.3 kDa
AP Mol Mass:
17 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
