Human Interleukin 22 Receptor Alpha 2 (IL-22Ra2) ELISA Kit
The Human Interleukin-22 Receptor Alpha-2 (IL22RA2) ELISA Kit is a powerful tool for the precise measurement of IL22RA2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results that are suitable for a wide range of research applications.IL22RA2 is a key receptor involved in the signaling pathway of interleukin-22, a cytokine that plays a crucial role in immune response and inflammation. Dysregulation of IL22RA2 has been linked to various autoimmune diseases, inflammatory conditions, and cancer, highlighting its importance as a potential therapeutic target and biomarker for disease diagnosis and prognosis.
With the Human IL22RA2 ELISA Kit, researchers can gain valuable insights into the role of IL22RA2 in health and disease, advancing our understanding of immune regulation and potentially leading to the development of novel treatment strategies. Order this reliable and easy-to-use kit today to accelerate your research efforts in the field of immunology and related disciplines.
Product Name:
Human Interleukin 22 Receptor Alpha 2 (IL-22Ra2) ELISA Kit
Product Code:
HUDL01497
Size:
96 Assays
Target:
IL22Ra2
Synonyms:
CRF2-S1, IL-22BP, ZcytoR16, Cytokine receptor class-II member 10, Cytokine receptor family type 2, soluble 1, Interleukin-22-binding protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
