IL21 belongs to the IL-15/IL-21 family. It is a cytokine with immunoregulatory activity. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. IL21 is expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. It may promote the transition between innate and adaptive immunity. IL-21 has been tried as therapy for alleviating allergic responses. It can significantly decrease pro-inflammatory cytokines produced by T cells in addition to decreasing IgE levels in a mouse model for rhinitis (nasal passage inflammation)Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Human Interleukin-21/IL-21 Recombinant Protein (RPES1853)
Product Code:
RPES1853
Size:
5µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Interleukin-21,IL-21, Za11, IL21
Accession:
Q9HBE4-1
Sequence:
Gln 23-Ser 155
Fusion tag:
Activity:
1. Measured by its ability to bind human IL-21R in a functional ELISA.2. Measured by its ability to induce Interferon-gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.4-2 ng/ml.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the mature form of human IL-21 isoform 1 (Q9HBE4-1) (Gln 23-Ser 155) was expressed and purified, with an initial Met.
Purity:
> 92 % as determined by reducing SDS-PAGE.
Mol Mass:
15.6 kDa
AP Mol Mass:
17 kDa
Formulation:
Lyophilized from sterile 20mM NaAc, 150mM NaCl, pH 5.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
