Human Interleukin 18 Receptor 1 (IL-18R1) ELISA Kit
The Human Interleukin-18 Receptor 1 (IL18R1) ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of IL18R1 levels in human serum, plasma, and cell culture supernatants. IL18R1 is a key receptor involved in the immune response, specifically in the regulation of cytokine activity and inflammation.This ELISA kit offers reliable and reproducible results, making it an invaluable tool for research applications focused on studying the role of IL18R1 in various diseases and disorders.
By accurately quantifying IL18R1 levels, researchers can gain a better understanding of its impact on conditions such as autoimmune disorders, inflammatory diseases, and cancer.Overall, the Human IL18R1 ELISA Kit provides a precise and efficient method for studying the function of IL18R1, allowing for the identification of potential therapeutic targets and the development of novel treatment strategies.
Product Name:
Human Interleukin 18 Receptor 1 (IL-18R1) ELISA Kit
Product Code:
HUDL01479
Size:
96 Assays
Target:
IL18R1
Synonyms:
CDw218a, CD218a, IL18-R1, IL-1Rrp, IL1RRP, CD218 antigen-like family member A, IL1 receptor-related protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
