Human Interleukin 13 Receptor Alpha 2 (IL-13Ra2) ELISA Kit

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SKU:
HUDL01468
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q14627
Sensitivity:
0.129ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CD213A2,CD213-A2,IL13R-A2,IL13-RA2,IL13-R,IL13-BP
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Frequently bought together:

Description

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Human Interleukin 13 Receptor Alpha 2 (IL-13Ra2) ELISA Kit

The Human Interleukin 13 Receptor Alpha 2 (IL13RA2) ELISA Kit is a powerful tool for the accurate detection of IL13RA2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research studies.IL13RA2 is a critical protein involved in the regulation of interleukin signaling and immune responses. It plays a key role in conditions such as asthma, allergy, and certain cancers, making it a valuable biomarker for understanding these diseases and exploring potential therapeutic strategies.

With the Human Interleukin 13 Receptor Alpha 2 (IL13RA2) ELISA Kit, researchers can confidently analyze IL13RA2 levels in human samples, advancing our understanding of disease mechanisms and paving the way for the development of targeted treatments.

Product Name:Human Interleukin 13 Receptor Alpha 2 (IL-13Ra2) ELISA Kit
Product Code:HUDL01468
Size:96 Assays
Target:IL13Ra2
Synonyms:CD213A2, CD213-A2, IL13R-A2, IL13-RA2, IL13-R, IL13-BP
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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