Human Interferon Gamma Induced Protein 10kDa (IP10) ELISA Kit

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SKU:
HUDL01544
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P02778
Sensitivity:
12.4pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
CXCL10,C7,IFI10,INP10,SCYB10,Crg2,GIP10,Mob1,Chemokine C-X-C Motif Ligand 10,Small Inducible Cytokine Subfamily B(Cys-X-Cys)Member 10
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Frequently bought together:

Description

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Human Interferon Gamma Induced Protein 10kDa (IP10) ELISA Kit

The Human Interferon-Gamma Induced Protein 10kDa (IP-10) ELISA Kit is specifically designed to accurately quantify levels of IP-10 in human serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.IP-10 is a key protein induced by interferon-gamma, playing a critical role in the immune response against viral and bacterial infections.

It is also involved in inflammatory conditions, autoimmune diseases, and cancer, making it a valuable biomarker for studying these conditions and developing targeted therapies.With its reliable performance and versatility, the Human IP-10 ELISA Kit is an essential tool for researchers investigating the role of IP-10 in various diseases and biological processes.

Product Name:Human Interferon Gamma Induced Protein 10kDa (IP10) ELISA Kit
Product Code:HUDL01544
Size:96 Assays
Target:IP10
Synonyms:CXCL10, C7, IFI10, INP10, SCYB10, Crg2, GIP10, Mob1, Chemokine C-X-C Motif Ligand 10, Small Inducible Cytokine Subfamily B(Cys-X-Cys)Member 10
Detection Method:Competition
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.Aspirate and wash 3 times
4.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.Aspirate and wash 5 times
6.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.Add 50µL Stop Solution. Read at 450 nm immediately.

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