Human Inter-Alpha-Trysin Inhibitor Heavy Chain H4 (ITIH4?ELISA Kit

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SKU:
HUDL01578
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
B2RMS9
Sensitivity:
1.46ng/mL
Range:
3.125-200ng/mL
ELISA Type:
Sandwich
Synonyms:
IHRP,PK120,H4P,ITIHL1,Plasma Kallikrein-Sensitive Glycoprotein,Plasma kallikrein sensitive glycoprotein 120,Inter-alpha-trypsin inhibitor family heavy chain-related protein
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Frequently bought together:

Description

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Human ITIH4 ELISA Kit

The Human Inter-alpha Trysin Inhibitor Heavy Chain H4 (ITIH4) ELISA Kit is specially designed for the accurate detection of ITIH4 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an ideal tool for a variety of research applications.ITIH4 is a key protein involved in the inhibition of trypsin activity, playing a crucial role in various physiological processes.

Dysregulation of ITIH4 has been linked to conditions such as inflammation, cancer, and liver diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human Inter-alpha Trysin Inhibitor Heavy Chain H4 (ITIH4) ELISA Kit offers researchers a reliable and effective method for measuring ITIH4 levels in human samples, providing valuable insights into its role in health and disease.

Product Name:Human Inter-Alpha-Trysin Inhibitor Heavy Chain H4 (ITIH4?ELISA Kit
Product Code:HUDL01578
Size:96 Assays
Target:ITIH4
Synonyms:IHRP, PK120, H4P, ITIHL1, Plasma Kallikrein-Sensitive Glycoprotein, Plasma kallikrein sensitive glycoprotein 120, Inter-alpha-trypsin inhibitor family heavy chain-related protein
Detection Method:Sandwich
Range:3.125-200ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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