The Human INS (Insulin) CLIA Kit is specifically designed for the quantitative detection of insulin levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reliable results for a variety of research applications.Insulin is a key hormone involved in regulating blood sugar levels and metabolism in the body. Changes in insulin levels can indicate conditions such as diabetes, metabolic disorders, and insulin resistance.
Studying insulin levels can help researchers understand these conditions better and develop targeted treatments.The Human INS (Insulin) CLIA Kit from Assay Genie is a valuable tool for researchers looking to investigate insulin-related pathways and diseases. With its ease of use and exceptional performance, this kit is a must-have for any laboratory conducting insulin research.
Product Name:
Human INS (Insulin) CLIA Kit
SKU:
HUES00915
Target:
Human INS (Insulin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human INS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human INS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human INS. You can calculate the concentration of Human INS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
94-109
98-113
Average (%)
106
100
106
1:4
Range (%)
87-98
96-110
88-102
Average (%)
92
103
94
1:8
Range (%)
103-118
90-104
98-114
Average (%)
109
96
105
1:16
Range (%)
93-104
93-109
97-109
Average (%)
98
99
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
96
EDTA plasma (n=5)
85-99
92
Cell culture media (n=5)
97-112
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.83
6.14
14.27
1.98
6.69
14.09
Standard deviation
0.19
0.56
0.94
0.23
0.74
0.92
C V (%)
10.38
9.12
6.59
11.62
11.06
6.53
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human INS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human INS in samples. No significant cross-reactivity or interference between Human INS and analogues was observed.
